UPLC-MS/MS同时检测苹果及其制品中的7种真菌毒素

摘要

A rapid and accurate method was developed for the determination of 7 mycotoxins (PAT,OTA,AOH,AME,ALT,TEN and TeA) in apple,apple juice,apple wine,apple acid,apple jam and canned apples by UPLC-MS/MS.10 g of the sample(10 mL of liquid sample) was precisely weighed,and extracted with acetonitrile containing 10 mmol/L citric acid solution,then 4 g of 4A molecular sieve and 1 g of NaC1 were added.After oscillation and centrifugation,the liquid supernatant was purged with C18 decontaminant.Finally,it was filtered through a 0.22 μm PTFE membrane and analyzed by UPLC-MS/MS.The method showed good linear relationships for 7 mycotoxins in the concentration range of 2-150 μg/L with correlation coefficients above 0.99.The detection limits were in the range of 0.02-1.8 μg/L,and the limits of quantitation were 1-5 μg/L.The recoveries of 7 mycotoxins were between 71.8％ and 112.4％ at three spiked concentrations levels of 10,50,100 μg/L.This method is rapid and efficient in the simultaneous detection of 7 mycotoxins in apple and its products.This will lay a foundation for the risk monitoring of mycotoxin contamination in apple and its products.%建立了苹果及5种苹果制品中7种真菌毒素(PAT,OTA,AOH,AME,ALT,TEN,TeA)的超高效液相色谱-串联质谱检测方法.准确称取苹果及5种苹果制品各10 g(液体样品量取10 mL),加入10 mmol/L柠檬酸乙腈溶液进行提取,再加入4 g4A型分子筛和1 g氯化钠除水盐析,离心后上清液采用C18净化,最后采用0.22 μm聚四氟乙烯膜(PTFE)过滤进行UPLC-MS/MS检测.7种真菌毒素在2～150 μg/L范围内线性关系良好(r2＞0.99);检出限为0.02 ～ 1.8 μg/L,方法定量下限为1～5μg/L.以10,50,100 μg/L浓度水平做加标回收实验,回收率为71.8％ ～112.4％.此方法快速、高效,为苹果及其制品中真菌毒素污染的风险监测奠定了基础.