基于Au纳米颗粒/还原氧化石墨烯C-反应蛋白免疫传感器的研制

摘要

In this paper,Au nanoparticles (Au NPs)/reduced graphene oxide (RGO) was used as the matrix of immunosensor to immobilize c-reactive protein (CRP) antibody on the surface of glassy carbon electrode.A sandwich immunosensor was constructed using quinone dicarboxylic acid as tag.Under the optimal experiment conditions,the concentration of CRP was detected by differential pulse voltammetry.The linear range is 0.25-100 ng/mL with a detection limit of 0.08 ng/mL and a correlation coefficient of 0.997.This immunosensor provides a new simple means for the determination of c-reactive protein.%采用还原氧化石墨烯-金纳米颗粒(RGO-Au NPs)作为免疫传感器的固定基质,将C-反应蛋白(CRP)抗体固定在玻碳电极表面,用蒽醌二羧酸作为标记物,制成夹心型的CRP免疫传感器.在最优实验条件下,通过示差脉冲伏安法对CRP的含量进行检测.该传感器在0.25～ 100 ng/mL范围内具有良好的线性关系,检出限为0.08 ng/mL,线性系数为0.997.该传感器为C-反应蛋白的检测提供了一种新的手段.