亲水作用色谱在糖类化合物分析中的应用

摘要

The analysis of carbohydrate structure and composition plays a vital role in the study of structure-activity relationship of carbohydrates.Hydrophilic interaction chromatography (HILIC) could be used to separate polar compounds like carbohydrates with satisfactory resolution.The stationary phase and retention mechanism of HILIC column commonly used in carbohydrates analysis were introduced.The effects of strong elution solvent ratio,mobile phase pH value,buffer salt concentration and column temperature on separation were also discussed.Application examples in analysis of monosaccharide composition,disaccharide composition of glycosaminoglycans,polymerization degree of oligosaccharides,glycosides,glycolipids,sugar alcohols and N-/O-glycans by HILIC were illustrated in this paper.%糖类化合物的结构和组成分析对于探究糖的结构与功能的关系具有重要作用.亲水作用色谱(HIL-IC)对糖类等极性化合物具有良好的分离效果.该文介绍了适合糖类分析的常用HILIC柱固定相及其分离机制,论述了强洗脱溶剂比例、流动相pH值、缓冲盐浓度和色谱柱温度对HILIC分离效果的影响,并举例说明了HILIC法在单糖组成、糖胺聚糖二糖组成、寡糖聚合度、糖苷、糖脂、糖醇以及N-/O-糖链分析中的应用.