NDRG1及NDRG2基因在结直肠癌组织中的表达及临床意义

摘要

目的:探讨NDRG1及NDRG2基因在结直肠癌中的表达与临床病理特征及预后的关系.方法:采用免疫组织化学检测92例结直肠癌组织及其配对癌旁组织中NDRG1及NDRG2蛋白的表达情况,统计学分析其与结直肠癌临床病理特征及预后的关系.结果:(1)NRDG 1及NDRG 2在92例结直肠癌组织中均有不同程度的表达:NRDG 1的阳性率为20.7％,NDRG 2的阳性率为23.9％,均明显低于其配对癌旁正常组织,差异有统计学意义(P＜0.01).(2)肿瘤浸润程度高(T3、T4)的结直肠癌组的NDRG1阳性率明显低于肿瘤浸润程度低(T1、T2)的结直肠癌组的阳性率(P＜0.05),差异有统计学意义.其阳性表达与病人的年龄、性别、肿瘤的分化程度、分期及有无淋巴结转移无关(P＞0.05);有淋巴结转移的结直肠癌组的NDRG2的阳性率明显低于无淋巴结转移的结直肠癌组的阳性率(P＜0.05),差异有统计学意义;其阳性表达与病人的年龄、性别,肿瘤的分期、分化程度、浸润程度无关(P＞0.05).(3)NDRG 1及NDRG 2表达水平与结直肠癌病人3 a生存率及5 a生存率无明显相关,无统计学意义.(4)结直肠癌中NDRG1及NDRG2无明显相关性(P＞0.05).结论:NDRG 1及NDRG2基因在结直肠癌的发展、侵袭及转移过程中起到重要作用,对临床诊断结直肠癌病情发展程度具有一定的参考意义.%Objective: To explore the expression of tumor-related genes of NDRG1 and NDRG2 in colorectal adenocarcinomas and the relationship with clinical pathological features and prognosis.Methods: Immunohistochemical two-staged methods were used to detected NDRG1 and NDRG2 in 92 cases of colorectal adenocarcinomas and cases of colorectal adenocarcinomas accompanying with lymph node metastasis.Results: (1) The expression of NDRG1 and NDRG2 in colorectal adenocarcinomas was positive of different levels: The positive expression of NDRG1 is 20.7％ (19/92), The positive expression of NDRG2 is 23.9％ (22/92), both remarkably lower than that in oncopathogenesis (P ＜ 0.01). (2) For NDRG1, The positive expression of higher invasion (T3、T4) in colorectal adenocarcinomas was remarkably lower than that of low invasion (T1、T2) (P＜0.05).There was no significant difference among different ages, differentiation degrees, genders, stages or lymphatic invasion groups for NDRG1 expression (P＞0.05).The positive rate of NDRG2 accompanying with lymph node metastasis in colorectal adenocarcinomas was remarkably lower than that accompanying without lymph node metastasis (P ＜ 0.05).There was no significant TNM stages, ages, differentiation degrees, genders or lymphatic invasion difference among different groups (P＞ 0.05). (3) The expression of NDRG1 and NDRG2 had no statistical significance for three and five year survival of patients. (4) There was no remarkably correlation between NDRG1 and NDRG2 in colorectal adenocarcinomas (P＞0.05).Conclusion: NDRG 1 and NDRG2 play an important part in the progress of tumor development, invasion or metastasis.It provides the the guiding significance for the clinical diagnosis.