补肾祛痰活血汤对缺血再灌注损伤大鼠Occludin、 ZO-1蛋白的调节作用

摘要

〔Abstract〕 Objective To investigate the effect of Bushen Huoxue decoction on rat brain edema, blood brain barrier permeability, damage zone occlusal protein (occludin), zonula occludens (ZO-1) protein in pretreatment ischemia reperfusion injury rats. Methods Fifty SD adult male rats were divided into normal group, model group, sham operation group, treatment group, control group according to the random number table method, 10 rats in each group. The treatment group was given Bushen Huoxue decoction, while the control group was given Xiaoshuan Tongluo capsule. The normal group, model group, sham operation group were given the corresponding double distilled water. The model of cerebral ischemia-reperfusion injury was established by suture method. After 72 hours, the brain water content was determined by dry wet weight method to reflect the degree of brain edema. The damage zone occludin and ZO-1 protein expression were tested by immunohistochemical method, and the results were analyzed by correlation analysis. Results Compared with the normal group and sham operation group, the brain tissue water content in rats at 72 h of model group, treatment group and control group was increased, the occludin and ZO-1 protein expression in brain tissue damage zone were significantly decreased (P<0.01). The water content of brain tissue was positively correlated with the degree of brain tissue injury, ZO-1 and occludin protein were negatively correlated with the degree of brain tissue injury. Conclusion Bushen Quyu Huoxue decoction can inhibite the decrease of occludin and ZO-1 protein, inhibite the blood brain barrier permeability and relieve brain edema formation.%目的：观察补肾祛痰活血汤对预处理脑缺血再灌注损伤的大鼠脑组织水肿、血脑屏障通透性及损伤区咬合蛋白（Occludin）、闭锁小带蛋白（zonula occludens-1, ZO-1）的影响。方法 SD成年雄性大鼠50只,按随机数字表法分为正常组、模型组、假手术组、治疗组、对照组，每组10只。治疗组给补肾祛痰活血汤、对照组给消栓通络胶囊，正常组、模型组、假手术组给与相应量的双蒸馏水。采用线栓法复制脑缺血再灌注损伤模型，72 h后，干湿重法测定脑含水量反映脑水肿程度，用免疫组织化学方法测定损伤区Occludin、ZO-1蛋白的表达量，并对各结果进行相关性分析。结果与正常组、假手术组比较，模型组、治疗组、对照组72 h时间点大鼠脑组织含水量较正常组、假手术组要增高一些，脑组织损伤区周围Occludin、ZO-1蛋白表达明显降低(P<0.01)。脑组织含水量与脑组织损伤程度正相关，脑组织损伤区周围Occludin、ZO-1蛋白与脑组织损伤程度呈负相关。结论补肾祛痰活血汤对缺血性脑损伤发生后损伤区Occludin、ZO-1蛋白含量降低有抑制作用，抑制血脑屏障通透性增高,减轻脑水肿形成。