青光安Ⅱ号对慢性高眼压模型大鼠视网膜GSK-3β及β-catenin mRNA表达影响

摘要

Objective To observe the effect of Qingguangan Ⅱ decoction on expression of GSK-3β mRNA and β-catenin mRNA of retinal ganglion cells in chronic high intraocular pressure model rats. Methods 40 (80 eyes) male SD rats were randomly divided into 6 groups, that is: blank group (A), model group (B), Qingguangan Ⅱ low dose group ?, Qing-guangan Ⅱ medium dose group (D), Qingguangan Ⅱ high dose group (E), Yimaikang dispersible tablets group (F). The rats in group B, C, D, E, F were established the model of chronic high intraocular pressure by the method of cauterizing of su-perficial scleral vein. Tissues of eyes were obtained after intragastric administration for 2 weeks and 4weeks. The relative ex-pression of GSK-3β mRNA and β-catenin mRNA was investigated by qPCR. Results At the time-point of 2-week, GSK-3β mRNA and β-catenin mRNA in group B were statistically expressed comparing with other groups (P<0.05). Moreover, at the time-point of 4-week, GSK-3β mRNA and β-catenin mRNA in group C, D and E were statistically expressed comparing with group F (P<0.05). Besides, GSK-3β mRNA and β-catenin mRNA in group C and D were statistically expressed compar-ing with group E (P<0.05). Conclusion Qingguangan Ⅱ decoction and Yimaikang dispersion tablets can effectively inhibit the expression of GSK-3β and increase the expression of β-catenin to protect the optic nerve of rats with chronic high intraocu-lar pressure. The experiment preliminarily indicated that, in the protection of optic nerve of rats with chronic high intraocular pressure, the high dose of Qingguang'an Ⅱ was the better choice.%目的 观察青光安Ⅱ号方对慢性高眼压模型大鼠视网膜GSK-3β及β-catenin mRNA表达影响.方法 将40只(80眼)雄性SD大鼠随机分成6组,分别为:空白组(A),模型组(B)、青光安Ⅱ号方低剂量组(C)、青光安Ⅱ号方中剂量组(D)、青光安Ⅱ号方高剂量组(E)、益脉康分散片组(F).将B、C、D、E、F组大鼠采用烧灼巩膜表浅静脉法建立大鼠慢性高眼压模型.模型大鼠维持高眼压状态2月后开始干预,干预后2周、4周分别用qPCR检测大鼠视网膜GSK-3β 及 β-catenin mRNA的相对表达量.结果 2周后,与B组比较,各组GSK-3βmRNA表达降低而β-catenin mRNA表达升高,差异有统计学意义(P<0.05).4周后,与F组比较,C、D、E组GSK-3βmRNA降低,β-catenin mRNA表达升高,差异有统计学意义(P<0.05).与E组比较,C、D组GSK-3βmRNA降低而β-catenin mRNA表达升高,差异有统计学意义(P<0.05).结论 青光安Ⅱ号方及益脉康分散片均能抑制GSK-3βmRNA的表达,并增加β-catenin mRNA的相对表达量,对慢性高眼压大鼠视网膜神经节细胞有一定的保护作用;初步观察表明复方中药青光安Ⅱ号方在对慢性高眼压大鼠视神经的保护中,效果优于益脉康分散片,且以青光安Ⅱ号方高剂量最优.