自拟安眠方对小鼠自主活动及协同戊巴比妥钠睡眠实验的影响

摘要

目的 观察自拟安眠方对小鼠自主活动及协同戊巴比妥钠睡眠实验的影响.方法 (1)将69只小鼠随机分为六组(空白对照组,西药对照组,中药对照组,安眠方低、中、高剂量组),空白对照组给予生理盐水,西药对照组给予地西泮,中药对照组给予甜梦胶囊,安眠方低、中、高剂量组分别给予12、38.7、120 g/(kg·d),观察10 min内各组小鼠活动次数及站立次数;(2)将90只小鼠,随机分为6组,空白对照组及西药、中药对照组同小鼠自主活动实验,安眠方低、中、高剂量组分别给予30.1、60.2、120 g/(kg·d),观察小鼠戊巴比妥钠睡眠潜伏期及睡眠时间.结果 与空白对照组相比,安眠方高剂量组小鼠活动次数与站立次数明显减少(P<0.01);协同戊巴比妥钠实验中安眠方中、高剂量组小鼠睡眠潜伏期缩短及睡眠时间延长与空白对照组相比较,差异均有统计学意义(P<0.05).结论 安眠方具有抑制小鼠自主活动的作用,具有缩短小鼠睡眠潜伏期及延长小鼠睡眠时间的作用.%Objective To investigate effects of Anmian decoction on automatic activity and pentobarbital sleep test of mice. Methods (1) 69 mice were divided into six groups (the control group, the Western medicine group, the traditional Chinese medicine group, Anmian Decoction low dosage group, Anmian Decoction medium dosage group and Anmian Decoc-tion high dosage group) for automatic activity test. The control group was given with normal saline, the West-ern medicine group was treated with diazepam, the traditional Chinese medicine group was treated with Tianmeng capsule, the low dosage, the medium dosage and the high dosage of Anmian decoction groups were separately taken Anmian dsecoction with 12, 38.7, 120.4 g/(kg·d) daily. Numbers of activity and stand of mice were observed in 10 minutes. (2) Another 90 mice were randomly divided into six groups in order to be tested by pentobarbital sleep test. All methods were same as automatic activity test except the low dosage, the medium dosage and the high dosage Anmian Decoction groups which were administrat-ed with 30.1, 60.2, 120.4 g/(kg·d) Anmian decoction every day, respectively. Results Numbers of activity and stand of mice in blank control group were significantly inhibited compared with the blank group (P<0.01). The medium and high dosages of Anmian decoction in pentobarbital sodium experiment could significantly shorten the latency of sleep and prolong the time of sleep in mice comparing with the control group (P<0.05). Conclusion Anmian decoction could inhibit the automatic activity of mice, decrease latency of sleep and prolong the time of sleep in mice.