电针对佐剂性关节炎大鼠关节滑膜细胞内TAK1表达的影响

摘要

〔Abstract〕 Objective To observe the effect of electroacupuncture on Zusanli, Guanyuan acupoints on expression of Transforming growth factor-βactivation of Protein kinase (TAK1) in articular synovial cell of rats with adjuvant-induced arthri-tis (AIA), and explore the possible intervention mechanisms of electroacupuncture. Methods 40 SD rats were randomly divided into blank group, model group, Methotrexate (MTX) group, the electroacupuncture group, 10 rats in each group. The A-IA model was made according to the Fueund's complete adjuvant (FCA) method. The electroapuncture group rats were given electroacupuncture at Zusanli and Guanyuan point for 20 minutes everyday, 3 weeks in all. The MTX group rats were treated with MTX suspension at the dosage of 0.35 mg/kg twice a week since the injection day, 3 weeks in total. The weight, paw volume and arthritis scores of rats were measured every 3 days. The TNF-α was detected by using radioimmunoassay. The expressions of TAK1, NF-κB in ankle synovial cell of rats were detected by Western Blot. The TNF-α, TAK1, NF-κB of each group were compared after intervention. Results Compared with the normal group, the body weight growth in model group was slow, the feet swelling was significantly severe, arthritis scores increased significantly (P<0.01) and the TNF-α significantly increased, TAK1 in synovial cell significantly increased, NF-κB expression significantly increased faster(P<0.01). Compared with the model group, the body weight in electropuncture group increased faster, the feet swelling was slighter, and arthritis scores got lower (P<0.05), the TNF-α ,TAK1 and NF-κB expression in synovial cells were lower (P<0.05). Conclusion Electroacupuncture on Zusanli, Guanyuan of AIA rats is effective, its intervention mechanism may be related to inhibiting the expression of TAK1 in synovial cells, then beneficial regulating on NF-κB the inflammatory signaling path-ways.%目的：观察电针足三里、关元穴对佐剂性关节炎（AIA）大鼠踝关节滑膜细胞内转化生长因子β激活激酶1（TAK1）表达的影响，探索电针对类风湿性关节炎（RA）可能的干预机制。方法40只SD大鼠随机分为空白组、模型组、甲氨蝶呤（MTX）组、电针组，每组10只。采用Fueund 氏完全佐剂(FCA)法造模。电针组予以电针大鼠关元穴、双侧足三里穴，MTX组予以0.35 mg/kg剂量的MTX灌胃治疗，空白组与模型组每日予以特制大鼠固定器固定后松绑放回笼内饲养。实验期间每3天1次测量大鼠体质量、足爪容积，并对关节炎指数进行评分；采用放射免疫法检测TNF-α、用Western Blot 检测TAK1、NF-κB蛋白表达。实验结束后比较各组大鼠踝关节滑膜滑膜组织中TNF-α，滑膜细胞内TAK1，胞核内NF-κB表达水平。结果与空白组比较，模型组大鼠体质量增长明显缓慢、足趾肿胀明显、关节炎指数明显升高（P<0.01），滑膜组织内TNF-α显著升高、滑膜细胞内TAK1含量显著升高、胞核内NF-κB表达显著升高（P<0.01）；与模型组比较，电针组大鼠体质量增长较快、足趾肿胀较轻、关节炎指数较低（P<0.05），滑膜组织中 TNF-α低于模型组、滑膜细胞内 TAK1、NF-κB 表达较低(P<0.05)。结论电针AIA大鼠足三里、关元等穴能够有效抗炎，其干预机制可能与抑制关节滑膜细胞内TAK1的表达，良性调控NF-κB这一炎症信号通路有关。