复方柴金解郁片抗抑郁作用的实验研究

摘要

Objective Preliminary study on the anti-depressant effect of compound Chaijin Jieyu tablet (CJJY). Methods Two kinds of mice models of depression were established, the mice forced swimming test and hanging tail test replication stress behavioral despair models of depression, serotonin acid and reserpine induced drug-induced depression model; detection of mouse forced swimming test and the tail suspension test in the time of immobility, serotonin acid induced depression in mice the number of head twitches, reserpine induced depression of body temperature, eyelid closure, akinesi-a difference degree of mice. Results Compared with the control group, the immobility time of 4 times and 2 times or (1 times) dose CJJY groups by the forced swimming test and tail suspension test in mice was significantly reduced (P<0.05). Compared with the control group, the head-twitching number of 4 times and 2 times dose CJJY group, in 5-HT acid in-duced head twitches test, was significantly increased (P<0.05 or P<0.01). In the experiment of depression induced by reser-pine, compared with the control group, the number of mice in the model group, which the eyelids were unable to open 1/2 and akinesia, was significantly increased. After 1h and 4 h, the body temperature of the rest mice was decreased (P<0.01 or P<0.05). Compared with model group, the number of mice in the CJJY 4 times and 2 times dose groups, which the eyelids were unable to open 1/2 and akinesia. was significantly decreased. After 1h and 4h, the body temperature of the rest mice in each group was increased (P<0.01 or P<0.05). Conclusion CJJY shows obvious anti-depression effect on all kinds of depression mouse models.%目的：初步探讨复方柴金解郁片的抗抑郁作用。方法建立两种小鼠抑郁模型，即小鼠强迫游泳实验和悬尾实验复制应激行为绝望抑郁模型，五羟色胺酸和利血平诱导药物所致抑郁模型；检测小鼠强迫游泳实验和悬尾实验不动时间，五羟色胺酸诱导的抑郁小鼠甩头次数，利血平诱导的抑郁小鼠体温、眼睑闭合、运动不能差异程度。结果小鼠强迫游泳实验和悬尾实验中，与空白对照组比较，复方柴金解郁片2倍或（1倍）剂量组不动时间明显减少（P＜0.05）。在五羟色胺酸诱导的小鼠甩头实验中，与空白对照组比较，复方柴金解郁片2倍剂量组甩头次数明显增加（P＜0.05或P＜0.01）。在利血平诱导的小鼠抑郁实验中，与空白对照组比较，模型组小鼠眼睑不能睁开1/2和运动不能动物数明显增加，1 h后及4 h后其余各组小鼠体温均有所下降(P＜0.01或P＜0.05)；与模型组比较，复方柴金解郁片2倍或1倍剂量组小鼠眼睑不能睁开1/2和运动不能动物数明显减少，药后1 h及4 h小鼠体温明显上升(P＜0.01或P＜0.05)。结论复方柴金解郁片对各种小鼠抑郁模型都有明显的抗抑郁作用。