山莓叶三萜对大鼠免疫功能的影响

摘要

To study the immunoregulatory actions of triterpene from Rubus corchorifolius Leaf (TRL), the immunodepressive mice model was constructed by intraperitoneal injection of cyclophosphamide (CTX). The model mice were randomly divided into high, middle, low dose groups and CK2 group which received 0.25, 0.50, 1.00 mg/Ml TRL and NaCl daily by gastric gavage respectively for 15 d. The weight of mice, the thymus index, the spleen index, the clearance index, the abdominal cavity macrophage engulfing rates, and the content of IL-2 and IL-6 in the serum were measured. The results showed that the high-dose TRL could improve the clearance index of the model mice. The IL-2 content in the middle dose group was lower than CK2( P ＜0.05), the high dose group was significantly lower than CK2 (P ＜0.01). The IL-6 content in the low dose group was lower than CK2, but the difference was not significant; the middle dose group and the high dose group were significantly lower than the low dose group and CK2( P ＜0.01), the high dose group was significantly lower than the other groups, except for the middle dose group( P ＜0.01).The results indicated that TRL had remarkable immunoregulatory actions.%为探明山莓叶三萜的免疫调节作用,腹腔注射环磷酰胺诱导大鼠免疫低下.将成模后大鼠分别连续灌服生理盐水和0.25、0.50、1.00 mg/mL山莓叶三萜精提物(以下简称模型对照组(CK2)、低浓度组、中浓度组和高浓度组),每天灌服1次,1 mL/次,连续15d.试验结果:各模型组大鼠的廓清指数和吞噬指数均随山莓叶三萜精提物浓度的升高呈上升趋势,但廓清指数各组间的差异不显著.中浓度组大鼠血清中的白细胞介素-2 (IL-2)含量显著低于CK2,高浓度组的极显著低于CK2.低浓度组大鼠血清中的IL-6含量低于CK2,但二者差异不显著；中浓度组和高浓度组均极显著低于低浓度组和CK2,高浓度组极显著低于除中浓度组外的其他模型组.结果表明,山莓叶三萜具有一定的免疫调节作用(山莓叶三萜提取物能提高大鼠巨噬细胞的吞噬能力；对大鼠IL-2、IL-6分泌具有抑制作用).