牛至挥发油正交提取工艺研究

摘要

Objectve To research the extraction process of Origannum vulgare L.,to determine the optimum extraction.Methods The essential oil of Origannum vulgare L.extracted by steam distillation,at the same time using GC with the major antibacterial component of p -cymene,thymol,carvacrol as a marked component for quality evaluation,optimal extraction process.Results The best extraction process of Origannum vulgare L.is:add 10 times the weight of water,Soak 30 minutes,Extraction of 6 hours,closed oil rate of 0.49％.Conclusion The essential oil of Origannum vulgare L.extracted by steam distillation,stable and viable.%目的 对牛至挥发油的正交提取工艺进行研究,确定最佳工艺条件.方法 采用水蒸气蒸馏法提取牛至挥发油,同时采用气相色谱法,以牛至主要抗菌成分对-聚伞花素、麝香草酚、香荆芥酚为标示成分,对牛至挥发油进行质量评价.结果 确定了牛至挥发油的最佳提取工艺:加药材重量的10倍量水,浸泡30min,提取6h,收油率为0.49％.结论 采用水蒸气蒸馏法提取牛至挥发油,稳定可行.