右归胶囊对肾阳虚大鼠免疫功能影响的实验研究

摘要

目的 观察右归胶囊对肾阳虚模型大鼠免疫功能的影响.方法 雄性SD大鼠分为正常组、模型组、干预组,采用肌肉注射氢化可的松建立肾阳虚模型,干预组给予右归胶囊混悬液灌胃.HE染色检测肾脏病理改变,测定尿液中17-羟皮质类固醇(17-OH-CS)含量,流式细胞检测血液中辅助性T细胞(CD4 +T)、Treg细胞(CD4+ CD25 +Tr)细胞等免疫细胞含量.结果 右归胶囊干预组尿17-OH-CS的含量与模型组比较有显著差异(P＜0.01)；模型组CD4 +T细胞数量显著减少,同时Treg细胞和CD8 +T细胞数量增加,与正常组比较差异有显著差异(P＜0.01),干预组CD4 +T细胞数量显著增加,同时Treg细胞和CD8+T细胞数量减少,与模型组比较差异有显著差异(P＜0.01).正常组大鼠肾脏结构正常,模型组肾脏发生了明显的病理变化,干预组的病理变化程度较模型组轻微.结论 右归胶囊可改善肾阳虚大鼠肾脏的病理结构,并通过影响外周血T淋巴细胞亚群,在一定程度上拮抗机体免疫功能低下,从而发挥改善肾阳虚作用.%Objective Yougui capsules of Kidney immune function and mechanism. Methods Male SD rats were divided into control group, model group,the intervention group,model group and the intervention group intramuscular injection of hydrocortisone kidney yang model control group and model group were given normal saline, the intervention group were given Yougui capsule suspension by gavage. HE staining of renal pathological changes, 17 - hydroxy corticosteroids (17 - OH - CS) content in the urine was measured by flow cytometry to detect blood helper T cells ( CD4 + T) , Treg cells ( CD4 + CD25 + Tr) content of the cells and other immune cells. Results Yougui capsule can increase the content of the kidney yang rat urine 17 - OH - CS, compared with the model group, the difference was statistically significant ( P < 0.01) ;model group,CD4 +Tcells,a significant reduction in the number of Treg cells at the same time and CD8 +Tcell numbers increase, compared with the control group,the difference was statistically significant (P <0. 01) ,low immune function. Intervention group,CD4 +Tcell count was significantly increased,while Treg cells and CD8 +Tcells decreased compared with the model group difference was statistically significant ( P < 0.01). The structure of the normal control group rat kidney, kidney pathological changes of the model group, the degree of pathological changes of the intervention group than in model group slightly. Conclusion Yougui capsule can improve the pathological structure of the rat kidney and kidney yang,and by influencing peripheral blood T lymphocyte subsets,antagonistic to some extent,immune dysfunction, kidney yang role to play to improve.