The hypoxic model to simulate hypoxic microenvironment in solid tumors was established and the effect of hydrocamptothecin(HCPT) on the hypoxia-induced over-expression of HIF-1α and VEGF genes was explored.Human cervical cancer SiHa cells were cultured in vitro under hypoxic conditions(37oC,5% CO2,1% O2) and treated with different concentrations of HCPT for 24 h.The mRNA and protein expression levels of HIF-1α,VEGF and Glut1 in SiHa cells were detected by semi-quantitative RT-PCR and Western blot respectively.Normoxic control groups were exposed to normoxic conditions for 24 h.Under normoxic conditions,HCPT had no obvious effects on the HIF-1α and VEGF gene expression.Hypoxia induced the up-regulation of HIF-1α protein and downstream VEGF gene,and HCPT showed a dose-dependently inhibitory effect on the hypoxia-induced over-expression of HIF-1α protein and VEGF gene expression in SiHa cells,whereas HCPT had no significant effect on the HIF-1α mRNA expression.No difference in HCPT cytotoxicity was observed between hypoxic groups and normoxic control groups.It was suggested that HCPT could inhibite the expression of HIF-1α protein and downstream VEGF gene in hypoxic SiHa cells in a dose-dependent manner,and the inhibitory effect was not related with HCPT cytotoxicity.
展开▼
机译:Research Progresses in the Inhibitory Effect of Bone Mesenchymal Stem Cell-Derived Exosome on Blood-Brain Barrier Disruption following Intracerebral Hemorrhage in Rats