基于荧光分析法的丁香酚提取工艺

摘要

We extract eugenol from Eugenia caryophyllata Thunb under different extraction condition.By comparing the fluorescence intensity at maximum emission wavelength 315 nm and excitation wavelengths at 280 nm,we find the effect is best by methanol,and methanol%几种不同提取条件下对丁香中丁香酚进行提取,比较提取后丁香酚溶液在发射波长为315 nm,激发波长为280 nm处的荧光强度,发现用无水甲醇作为溶剂提取效果最好,而且无水甲醇自身的荧光强度很弱,不影响用荧光分析方法对丁香酚定量研究;超声波提取方法效果最好,用无水甲醇做溶剂超声5 min即可从丁香药材中将丁香酚全部提取出.