玉米成熟胚愈伤组织诱导与继代培养的影响因素研究

摘要

Twenty-eight maize genotypes were selected as experimental materials to study the factors (culture medium type, genotype, disinfection time, cut pattern of embryo, temperature, sucrose concentration, CH concentration, 2,4-D concentration) affecting the induction and subculture of calli from mature embryo. The results showed that culture medium, CH concentration and 2,4-D concentration had no significant on callus induction, but had obvious effects on quality of calli in the course of subculture; Other five factors greatly affected the induction and subculture of calli. Three materials with high cultivating ability were selected, and the suitable media were initially established.%以28个不同基因型的玉米成熟胚为试验材料,初步研究了培养基种类、基因型、消毒时间、切胚方式、变温处理、蔗糖质量浓度、酸水解酪蛋白质量浓度、2,4-D质量浓度等因素对愈伤组织诱导与继代的影响.结果表明:培养基、酸水解酪蛋白质量浓度、2,4-D质量浓度等因素对愈伤组织诱导率的影响不显著,但对继代过程中愈伤组织质量影响较大;其余5个因素对愈伤组织诱导率的影响显著,并且对继代培养中的愈伤组织质量也有明显影响.最终选出了3个高培养力材料,初步确定了比较适合玉米成熟胚愈伤组织诱导与继代的培养基.