利用聚丙烯酰胺凝胶电泳鉴定壳斗科植物种质资源

摘要

The salt soluble proteins of five sample seeds were analyzed by plyaerylamide gel electrophoresis(PAGE),to provide scientific basis for the identification of the germplasm resource of Fagaceae.Using five sample seeds(including four species and two genera of the Fagaceae plants)as material,the salt soluble proteins were respectively extracted and detected by SDS-PAGE.The kinds of salt soluble protein of Castanea seguinii Dode were most abundant and had 19 strips,but Quercus acutissima Carr only had 11 strips.Comparison of the strips each other,the similar degree was highest between Castanea seguinii Dode and Castanea mollissima B1,followed by Castanea henryi (Skan) Rehd.et Wils and Quercus acutissima Carr,Castanea seguinii Dode and Castanea henryi(Skan) Rehd.et Wils.The great difference was found between Castanea mollissima Bl and Quercusacutissima Carr.This gave the evolution relation:Quercus acutissima Carr→Castanea henryi (Skan) Rehd.et Wils → Castanea seguinii Dode→ Castanea mollissima B1.Also,this showed that SDS-PAGE could identify evolution relation and phylogenetic relation among the Fagaceae plants.%通过对5个壳斗科植物盐溶蛋白的聚丙烯酰胺凝胶电泳(PAGE)分析,为壳斗科植物的种质资源鉴定提供科学依据.以栗属(板栗、锥粟、茅栗)和栎属(麻栎)5个壳斗科植物为试验材料,分别提取种子内盐溶蛋白,并进行PAGE电泳检测.结果表明,茅粟(Castanea seguinii Dode)的盐溶蛋白种类最丰富,有19条,而麻栎(Quercus acutissima Carr)最少,有11条.对每条谱带进行相似性比较,发现茅栗与板栗(Castanea mollissima Bl)的相似程度最高,其次是锥栗[Castanea henryi(Skan)Rehd.et.Wils]和麻栎,再次是茅栗与锥栗,而板栗与麻栎的差异性最大.从而推断出它们之间进化关系:麻栎→锥栗→茅栗→板栗.袁明利用SDS-PAGE技术可用于鉴定壳斗科植物之间的进化关系和亲缘关系.