为建立高效稳定的小麦遗传转化体系,以小麦郑麦9023的幼胚为外植体诱导愈伤组织,研究了培养基中不同质量浓度2,4-D、KT对愈伤组织诱导和愈伤组织继代培养的影响,进而完成整个植株再生的进程.结果表明:以小麦郑麦9023的幼胚作为外植体来诱导愈伤组织,诱导效果最好的培养基是含有3.0 mg/L 2,4-D的MS培养基,出愈率最高达92.5%;愈伤组织进行继代培养的最佳培养基是含有1.0 mg/L 2,4-D 和0.1 mg/L KT的MS培养基;最佳的悬浮培养基是含有1.0 mg/L 2,4-D和0.1 mg/L KT的MS培养基;最佳的悬浮系继代时间为16 d;悬浮系中的成熟胚在基本培养基上可以发育成正常的植株.所建立的悬浮系可以用来进行小麦基因工程遗传改良和体细胞无性系突变体的筛选.%In order to establish a rapid,efficient and stable genetic transformation system for wheat,rataria of wheat Zhengmai 9023 were cultured to induce calluses,and the effect of different concentrations of 2,4-D and KT in mediums on callus induction and subculture was studied.The results showed that the optimal callus induction medium was MS+ 3.0 mg/L 2,4-D,and the highest ratio of callus induction was 92.5%;the optimal medium for callus subculture was MS+ 1.0 mg/L 2,4-D+0.1 mg/L KT;the optimal medium for cell suspension was MS +1.0 mg/L 2,4-D + 0.1 mg/L KT;the optimal time for subculture of suspension system was 16 d.The mature embryos in suspension system could develop into normal plants in the basic medium.The embryogenic cell suspension could be used for genetic engineering and screening of somaclonal mutants in wheat.
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