头孢青霉素和羧苄青霉素对大豆子叶节茎再生的影响

摘要

试验通过向茎诱导培养基和茎伸长培养基中添加不同浓度的头孢青霉素（Cef）和羧苄青霉素（Cb）来研究二者对大豆子叶节茎再生潜在的影响。一定浓度的Cef或Cb对大豆子叶节部位茎的再生有促进或抑制作用。不同基因型大豆的最适Cef或Cb添加浓度不同。培养基中添加100 mg.L-1 Cef或120 mg.L-1 Cb能显著提高垦农18号（K18）子叶节部位丛生芽的诱导及可伸长茎的生成和伸长;150 mg.L-1 Cef对垦农4号（K4）子叶节部位茎的再生有显著的促进作用1,20 mg.L-1 Cb和200 mg.L-1 Cb分别显著提高K4丛生芽的再生和随后可伸长茎的生成及伸长。经121℃高压灭菌处理的Cef和Cb对大豆子叶节部位丛生芽的诱导和可伸长茎的生成及伸长有显著的抑制作用。%The influences of cefotaxime and carbenicillin on shoot regeneration potential of soybean cotyledonary-node were investigated by supplying with various concentrations of cefotaxime and carbenicillin to shoot induction medium and shoot elongation medium.The capability of shoot regeneration from soybean cotyledonary-node could be promoted or suppressed by supplying with some certain concentration of cefotaxime and carbenicillin.However,the most appropriate treatment is genotype-dependent.The buds regeneration,shoot multiplication and elongation of cotyledonary-node of K18 can be enhanced significantly by supplying with 100 mg·L-1 cefotaxime or 120 mg·L-1 carbenicillin in culture medium;the capability of shoot regeneration of K4 could be promoted with 150 mg·L-1 cefotaxime or 120 mg·L-1 carbenicillin on buds regeneration and 200 mg·L-1 on shoot multiplication and elongation.Autoclaved cefotaxime and carbenicillin showed detrimental effects on buds regeneration and shoot multiplication and elongation respectively.