高效液相色谱法测定仙鹤草中鹤草酚含量

摘要

目的　分离与测定仙鹤草中鹤草酚的含量。方法　用高效液相色谱法测定鹤草酚的含量，柱规格Kmmasil ODS柱(5 μm, 15 cm×4.6 mm i.d.), 流动相组成为甲醇-水（75∶25）,检测波长λ=292 nm。结果　测定鹤草酚的平均回收率为 97.15 %, 相对标准偏差为 0.96 % (n=6)。结论　本方法用于仙鹤草中鹤草酚的测定，结果十分理想。%Objective　To separate and determine agrimophol in agrimonia pilosa ledeb.Methods　Agrimophol was determined by high performance liquid chormatography with a column of Kmmasil ODS (5 μm, 15 cm×4.6 mm i.d.). The mobile phase was MeOH∶H2O (75∶25),λ=292 nm.Results　The average recovery and RDS of agrimophol was 97.15 % and 0.96 % (n=6).Conclusion　This method could be used for determination of agrimophol in agrimonia pilosa ledeb effectively.