Lifeceramics 对长期饮酒大鼠肝脏的保护作用

摘要

Objective To investigate protective effects of Lifeceramics(LC)on the liver of rats with chronic alcoholism.Methods Male SD rats were randomly divided into four groups:blank control group,alcohol (liquor)alone-administered group,LC alone-administered group, liquor+ LC-administered group,12 rats in a group.The rats of different groups were orally administered by gavage with test substance according to 10 mL/kg body mass;30 min later,oral administration of wine once a day for four weeks.At the end of the administration experiments, the activities of alanine aminotransferase(ALT)and aspartate aminotransferase(AST)in serum were detected.The content of malondialdehyde (MDA)and glutathione (GSH)in livers were examined.The activities of alchol dehydrogenase (superoxied dismutase,ADH ),acetaldehyde dehydrogenase(ALDH),glutathione peroxidase(GSH-Px)and superoxied dismutase(SOD)in livers were estimated.Results LC significantly reduced serum ALT and AST activity of liquor drinking rats,improved liver ADH,ALDH,GSH-Px activity and GSH content,reduced MDA content.Conclusion LC has protective effects on alcohol-induced liver injury.%目的：观察 Lifeceramics （LC）对长期饮酒导致的大鼠肝脏功能损伤的改善作用。方法将 SD大鼠随机分为4组：空白对照组、饮酒组、对照组（LC 组）以及 LC＋饮酒组，每组12只。各组大鼠按10 mL／kg 体质量灌胃给予受试物。30 min 后再灌胃给予白酒，每天1次，共4周。实验结束后，分别检测大鼠血清中丙氨酸转氨酶（alanine aminotransferase，ALT）和天冬氨酸转氨酶（aspartate aminotransferase，AST）活性，检测大鼠肝脏中谷胱甘肽（glutathione，GSH）和丙二醛（malondialdehyde，MDA）含量，以及乙醇脱氢酶（alchol dehydrogenase，ADH）、谷胱甘肽过氧化物酶（glutathione peroxidase，GSH-Px）、乙醛脱氢酶（acetaldehyde dehydrogenase，ALDH）、超氧化物歧化酶（superoxied dismutase，SOD）活性。结果 LC 能显著降低饮酒大鼠血清 ALT 和 AST 活力，提高肝脏 ADH、ALDH、GSH-Px 活力和 GSH 含量，降低 MDA 含量。结论 LC 对长期饮酒大鼠肝脏具有显著的保护作用。