无创性延迟肢体缺血预适应对大鼠脑缺血再灌注损伤的保护作用

摘要

目的 观察无创性肢体缺血预适应(noninvasive delayed limbischemic preconditioning,NDLIP)对大鼠脑缺血再灌注损伤的保护作用.方法 将30只健康雄性SD大鼠随机分成假手术组、缺血再灌注组和NDLIP组各10只.NDLIP组大鼠给予左侧后肢缺血5 min、再灌注5 min,每天1次,连续操作3d,然后再制作缺血模型;假手术组大鼠给予同样的麻醉和操作,但不进行颈总动脉的闭塞;假手术组和缺血再灌注组不进行NDLIP操作.检测各组大鼠海马超氧化物歧化酶(superoxide dismutase,SOD)、丙二醛(malondialdehyde,MDA)含量和Bax、Bcl 2、Caspase-3蛋白表达水平.结果 缺血再灌注组和NDLIP组大鼠海马MDA含量明显高于假手术组,SOD含量明显低于假手术组,Bax、Caspase-3表达水平明显高于假手术组,Bcl-2表达水平明显低于假手术组;NDLIP组大鼠海马MDA含量明显低于缺血再灌注组,SOD含量明显高于缺血再灌注组,Bax、Caspase-3表达水平明显低于缺血再灌注组,Bcl-2的表达水平明显高于缺血再灌注组.结论 NDLIP能显著减少脑缺血再灌注损伤后凋亡的发生和氧化应激损伤,对脑缺血再灌注损伤具有保护作用.%Objective To study the protective effectof noninvasive delayed limb ischemia preconditioning(NDLIP) on cerebral ischemia reperfusion injury in rats.Methods Thirty healthy male SD rats randomly divided into 3 groups,Sham-operated group,ischemic-reperfusion group and NDLIP group(n =10/group).Rats in NDLIP group were subjected to 5 minutes ischemia/5 minutes reperfusion on the left hind limb once a day for 3 consecutive days,then all rats were subjected to cerebral ischemia reperfusion injury.The Sham-operated group gave the same anesthesia an.d operation,but did not carry out the occlusion of the common carotid artery.The sham-operated group and ischemic-reperfusion group were not performed NDLIP operation.The expression of superoxide dismutase(SOD) and malondialdehyde(MDA) was detected by the kit.The protein expression of Bax,Bcl-2 and Caspase-3 were detected by Western blotting.Results Compared with the rats in the Sham-operation group,the contents of the hippocampal MDA,Bax and Casaspe-3 were significantly higher in the ischemic-reperfusion group.Compared with the rats in the ischemic-reperfusion group,the contents of the hippocampal MDA,Bax and Casaspe3 were significantly decreased in the NDLIP group.Compared with the rats in the Sham-operation group,the contents of the hippocampal SOD and Bcl-2 were significantly decreased in the ischemic-reperfusion group.Compared with the rats in the ischemic-reperfusion group,the contents of the hippocampal SOD and Bcl-2 were significantly increased in the NDLIP group.Conclusion NDLIP could significantly reduce the occurrence of apoptosis and oxidative stress damage after cerebral ischemia reperfusion injury,it has protective effect on cerebral ischemia reperfusion injury.