番茄红素粗提液中质量浓度测定方法的研究

摘要

This paper studied on the determination influence of lycopene content in unpurified extract by different testing methods. Lycopene was extracted in dark by acetone, ethyl acetate and hexane in the low temperature. Lycopene was scanned in 300 ~ 600 nm by UV - vis spectrophotometer and detected content by HPLC. The spectra of acetone unpurified extract in 300 ~ 600 nm was a wide peak form, but UV - visible spectra of ethyl acetate and hexane were similar to the spectra of lycopene standard substance. It was proved that acetone unpurified extract contained lycopene by HPLC. lycopene contents in the same acetone unpurified extract were 4.76 mg/mL and 2. 19 mg/mL, detected by UV spectrophotometry and HPLC respectively. The extraction method did not influence the UV - visible spectra of acetone unpurified extract. Lycopene content in acetone unpurified extract cannot be detected by UV spectrophotometry.%研究不同测定方法对番茄红素粗提液质量浓度测定的影响.以丙酮、乙酸乙酯和正己烷在低温、避光条件下提取番茄红素,并在300～600 nm区间进行紫外可见光谱扫描,再用HPLC法测定粗提液中番茄红素质量浓度.丙酮粗提液300～600 nm区间的紫外可见吸收光谱图呈一宽峰,而乙酸乙酯和正己烷粗提液的紫外可见吸收光谱与番茄红素标准品相似.HPLC法证明丙酮粗提液中确实含有番茄红素.用紫外分光光度法和HPLC法测定同一丙酮粗提液中番茄红素质量浓度分别为4.76 mg/mL和2.19 mg/mL.番茄红素丙酮粗提液的紫外吸收光谱图呈一宽峰并非受浸提方法的影响,可能与浸提溶剂有关,因此番茄红素的丙酮粗提液不能用紫外分光光度法进行质量浓度测定.