以自制磁性琼脂糖微球为基质，环氧氯丙烷为活化剂，亚氨基乙二酸作为螯合剂，制备了表面螯合Ni2＋的磁性凝胶微球（Mag-Agarose-Ni）．IR结果表明Ni2＋成功螯合到了磁性凝琼脂糖胶微球上；SEM 结果显示在螯合Ni2＋后，M ag-Agarose-Ni形貌没有发生明显变化，且平均粒径为23μm ；原子吸收光谱结果表明M ag-Agarose-Ni表面螯合的Ni2＋的量为2．12×10-5 mol/mg ；磁性能测试表明M ag-Agarose-Ni具有超顺磁性，其磁饱和强度为20．8 emu/g ，具有良好的磁响应性．将Mag-Agarose-Ni用于六聚组氨酸融合蛋白K8的纯化研究， SDS-PAGE结果表明 Mag-Agarose-Ni较市售 Ni-NTA对K8具有更优的亲和分离效果，经15 min的孵育后， Mag-Agarose-Ni对K8的吸附容量可达到8．8μg/mg ．%Magnetic Ni2+ chelate affinity agarose microspheres (Mag-Agarose-Ni) were prepared by introducing nickel ions into the surface of self-made Magnetic Agarose Microspheres with the aid of iminodiacetic acid as chelating agent .The results of Fourier transform infrared (FTIR) indicated that Ni2+ had successfully chelated on the magnetic agarose gel microspheres .The analysis of scanning electron micrographs (SEM ) showed that the size of Mag-Agarose-Ni had no significant change after the chelation with Ni2+ ,and the average particle diameter was 23 μm .The atomic absorption spectroscopy confirmed the amount of chelated Ni2+ on the surface of magnetic agarose microspheres was 2 .12 × 10-5 mol/mg .The magnetic measurement revealed that the resultant Mag-Agarose-Ni were superparamagnetic with a saturation magnetization of 20 .8 emu/g .Mag-Agarose-Ni was used as a magnetic affinity separation support for 6 × His-tagged Proteins K8 .The results of SDS-PAGE showed that Mag-Agarose-Ni had better affinity to K8 than commercial Ni-NTA , and the adsorption capacity of K8 by Mag-Agarose-Ni was 8 .8 μg/mg after 15 minutes incubation .
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