水飞蓟宾联合5-FU 对胃癌细胞株 MGC803恶性生物学行为的抑制作用研究

摘要

目的：：研究水飞蓟宾联合5-FU 处理对胃癌细胞株 MGC803恶性生物学行为的抑制作用.方法：培养胃癌细胞株 MGC803,分为 NC 组、5-Fu 组、SB＋5-Fu 组并用不同条件进行处理,检测凋亡细胞数目、侵袭细胞数目以及增殖、侵袭相关基因的表达量.结果：处理后6h、12h、18h、24h 时,5-Fu 组、SB＋5-Fu 组凋亡细胞的数目显著多于 NC 组、侵袭数目显著少于 NC 组,SB＋5-Fu 组凋亡细胞的数目显著多于5-Fu 组、侵袭数目显著少于5-Fu 组(P ＜0．05)；5-Fu 组、SB＋5-Fu 组细胞中 Vav3、PTP1B、GOLPH3、RUNX3、Sipa1、UbcH10、NEDD9、Mig-7、CD157、AEP、Galectin-1的 mRNA 含量低于 NC组(P ＜0．05)；SB＋5-Fu 组细胞中 Vav3、PTP1B、GOLPH3、UbcH10、NEDD9、Mig-7、CD157、AEP、Galectin-1的 mRNA 含量低于5-Fu 组(P ＜0．05),RUNX3、Sipa1的 mRNA 含量与5-Fu 组差异无统计学意义(P ＞0．05).结论：与5-FU 单药处理相比,水飞蓟宾联合5-FU 处理能够更为有效的促进胃癌细胞凋亡、抑制胃癌细胞侵袭、调节增殖和侵袭相关基因的表达.%Objective:To study the inhibitory effect of silibinin combined with 5-FU treatment on malignant biological behaviors of gastric cancer cell lines MGC803.Methods:Gastric cancer cell lines MGC803 were cultured,divided into NC group,5-Fu group and SB+5-Fu group and treated with different conditions,and then the number of apoptotic cells,the num-ber of invasive cells as well as the expression of proliferation and invasion-related genes were detected.Results:6 h,12 h,18 h and 24 h after treatment,the number of apoptotic cells of 5-Fu group and SB+5-Fu group was significantly more than that of NC group,the number of invasive cells was significantly less than that of NC group,the number of apoptotic cells of SB+5-Fu group was significantly more than that of 5-Fu group,and the number of invasive cells was significantly less than that of 5-Fu group;mRNA contents of Vav3,PTP1B,GOLPH3,RUNX3,Sipa1,UbcH10,NEDD9,Mig-7,CD1 57,AEP and Galectin-1 of 5-Fu group and SB + 5-Fu group were lower than those of NC group;mRNA contents of Vav3,PTP1B,GOLPH3, UbcH10,NEDD9,Mig-7,CD1 57,AEP and Galectin-1 of SB+5-Fu group were lower than those of 5-Fu group,and mRNA contents of RUNX3 and Sipa1 were not different from those of 5-Fu group.Conclusion:Compared with single 5-FU treatment, silibinin combined with 5-FU treatment can more effectively promote gastric cancer cell apoptosis,inhibit gastric cancer cell in-vasion and regulate the expression of proliferation and invasion-related genes.