吴茱萸碱对大肠癌细胞株 HT29恶性生物学行为及 Wnt/β-catenin 信号通路的调控作用

摘要

目的：：研究吴茱萸碱对大肠癌细胞株 HT29恶性生物学行为及 Wnt/β-catenin 信号通路的调控作用。方法：培养大肠癌细胞株 HT29,分为空白对照组和吴茱萸碱组,不同条件处理后测定细胞活力、不同细胞周期所占比例以及 VEGFA、VEGFB、VEGFC、MMP3、MMP14、Wnt、β-catenin 的含量。结果：(1)细胞活力：吴茱萸碱组的 MTT 值显著低于空白对照组；(2)细胞周期：吴茱萸碱组的 S 期、G2/M期比例均低于空白对照组,G0/G1期比例高于空白对照组；(3)VEGF 和 MMP 含量：吴茱萸碱组细胞中 VEGFA、VEGFB、VEGFC、MMP3和 MMP14的含量低于空白对照组；(4)Wnt/β-catenin 信号通路：吴茱萸碱组细胞中 Wnt、β-catenin 的含量低于空白对照组。结论：吴茱萸碱能够抑制大肠癌细胞株HT29的增殖过程并下调 VEGF、MMP 的表达,且这一作用可能通过抑制 Wnt/β-catenin 信号通路的激活来实现。%[ABSTRACT]Objective:To study the regulatory effect of evodiamine on the malignant biological behaviors and Wnt/β-catenin signaling pathway of colorectal cancer cell lines HT29.Methods:Colorectal cancer cell lines HT29 were cultured and divided into blank control group and evodiamine group,and after different treatment,cell viability,proportion of different cell cycle as well as the contents of VEGFA,VEGFB,VEGFC,MMP3,MMP14,Wnt andβ-catenin were detected.Results:(1) cell viability:MTT value of evodiamine group was significantly lower than that of blank control group;(2)cell cycle:propor-tion of both S phase and G2/M phase of evodiamine group were lower than those of blank control group,while proportion of G0/G1 phase was higher than that of blank control group;(3 )VEGF and MMP contents:VEGFA,VEGFB,VEGFC, MMP3 and MMP14 contents of evodiamine group were lower than those of blank control group;(4)Wnt/β-catenin signaling pathway:Wnt andβ-catenin contents of evodiamine group were lower than those of blank control group.Conclusion:Evodia-mine can inhibit the proliferation of colorectal cancer cell lines HT29 and down-regulate the expression of VEGF and MMP, and the effect may be achieved by inhibiting the activation of Wnt/β-catenin signaling pathway.