目的:研究泛素偶联酶2C (UBE2C )对神经母细胞瘤增殖、侵袭的调节作用.方法:培养S H-SY5Y神经母细胞瘤细胞株并随机分为 UBE2C-siRNA 组和NC-siRNA 组,分别转染 UBE2C的siR-NA和NC的siRNA .转染siRNA后24小时,抽提细胞内的RNA 并通过荧光定量PCR反应测定促增殖基因 YB-1 、CyclinD1 、CDK4 、Aurora-A 、Ki-67 及增殖抑制基因 LC3 、Beclin1 、GRP78 、IRE1α 、PERK 、ATF6 及侵袭基因 KLF4 、RIPK3 、HIF-1α 、Integrin β1 、MMP9 的 mRNA 表达量.结果:UBE2C-siRNA组细胞中YB-1 、CyclinD1 、CDK4 、Aurora-A 、Ki-67 、KLF4 、RIPK3 、HIF-1α 、Integrin β1 、MMP9的mRNA表达量显著低于NC-siRNA 组,LC3 、Beclin1 、GRP78 、IRE1α 、PERK 、ATF6的mR-NA表达量显著高于NC-siRNA组.结论:抑制 UBE2C基因能够调节神经母细胞瘤内增殖及侵袭基因的表达,进而阻碍细胞的增殖和侵袭.%Objective :To study the regulatory effect of ubiquitin-conjugating enzyme 2C (UBE2C) on the proliferation and invasion of neuroblastoma .Methods :SH-SY5Y neuroblastoma cell lines were cultured and randomly divided into UBE2C-siRNA group and NC-siRNA group that were transfected with UBE2C siRNA and NC siRNA respectively .24 hours after siR-NA transfection ,the RNA in the cells was extracted ,and fluorescence quantitative PCR reaction was used to detect the mRNA expression of pro-proliferation genes YB-1 ,CyclinD1 ,CDK4 ,Aurora-A and Ki-67 ,anti-proliferation genes LC3 ,Beclin1 , GRP78 ,IRE1α ,PERK and ATF6 as well as invasion genes KLF4 ,RIPK3 ,HIF-1α ,Integrin β1 and MMP9 .Results :YB-1 , CyclinD1 ,CDK4 ,Aurora-A ,Ki-67 ,KLF4 ,RIPK3 ,HIF-1α ,Integrin β1 and MMP9 mRNA expression in UBE2C-siRNA group of cells were significantly lower than those in NC-siRNA group whereas LC3 ,Beclin1 ,GRP78 ,IRE1α ,PERK and ATF6 mRNA expression were significantly higher than those in NC-siRNA group .Conclusion :Inhibition of the UBE2C gene can regulate the expression of proliferation and invasion genes in neuroblastoma to hinder cell proliferation and invasion .
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