Objective :To study the correlation of miR-106b in nasopharyngeal carcinoma with cell cycle progression and cell invasion .Methods :Patients who were diagnosed with nasopharyngeal carcinoma by pathological biopsy in Shenmu Hospital between March 2013 and February 2018 were included in the study ,and the nasopharyngeal carcinoma tissue and tissue adja-cent to nasopharyngeal carcinoma obtained from biopsy were collected ,miRNA was extracted to determine miR-106b expres-sion ,and RNA was extracted to determine the mRNA expression of cell cycle-related genes and cell invasion-related genes .Re-sults :miR-106b expression as well as FOXC1 ,CyclinD1 ,CyclinE ,TRIM27 ,uPAR ,PARP1 and MTA1 mRNA expression in nasopharyngeal carcinoma tissues were higher than those in adjacent tissues ,CYB5R2 ,p16 ,IRF5 and E-cadherin mRNA ex-pression were significantly lower than those in adjacent tissues whereas ;CYB5R2 ,p16 ,IRF5 and E-cadherin mRNA expres-sion in nasopharyngeal carcinoma tissues with high miR-106b expression were lower than those in nasopharyngeal carcinoma tissues with low miR-106b expression whereas FOXC1 ,CyclinD1 ,CyclinE ,TRIM27 ,uPAR ,PARP1 and MTA1 mRNA ex-pression were higher than those in nasopharyngeal carcinoma tissues with low miR-106b expression .Conclusion :The lowly ex-pressed miR-106b in nasopharyngeal carcinoma can promote the cell cycle progression and cell invasion in the lesion .%目的:研究鼻咽癌中miR-106b与病灶内细胞周期进程、细胞的侵袭的相关性.方法:选择2013年3月～2018年2月期间在神木市医院经病理学活检诊断为鼻咽癌的病例纳入研究,留取活检时得到的鼻咽癌组织和鼻咽癌旁组织,抽提miRNA 后测定miR-106b的表达量,抽提RNA 后测定细胞周期相关基因、细胞侵袭相关基因的 mRNA 表达量.结果:鼻咽癌组织内 miR-106b 的表达量以及FOXC1 、CyclinD1 、CyclinE 、TRIM27 、uPAR 、PARP1 、MTA1 的 mRNA 表达量高于鼻咽癌旁组织, CYB5R2 、p16 、IRF5 、E-cadherin的mRNA表达量显著低于鼻咽癌旁组织;miR-106b高表达的鼻咽癌组织内 CYB5R2 、p16 、IRF5 、E-cadherin 的 mRNA 表达量低于 miR-106b 低表达的鼻咽癌组织, FOXC1 、CyclinD1 、CyclinE 、TRIM27 、uPAR 、PARP1 、MTA1的 mRNA 表达量高于miR-106b低表达的鼻咽癌组织.结论:鼻咽癌中低表达的miR-106b对病灶内细胞周期的进程、细胞的侵袭均具有促进作用.
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