脓毒症大鼠模型心肌组织和肺组织中活性氧含量与组织损伤、细胞凋亡的相关性研究

摘要

目的:研究脓毒症大鼠模型心肌组织和肺组织中活性氧(ROS)含量与组织损伤、细胞凋亡的相关性.方法:选择SD雄性大鼠作为实验动物并分为两组,CLIP组以盲肠结扎加穿孔法建立脓毒症模型,Sham组进行假手术操作.造模后收集血清并测定心肌损伤及肺损伤标志物的含量,收集肺组织及心肌组织并测定ROS、MDA含量及细胞凋亡基因的表达量.结果:CLIP组大鼠心肌组织和肺组织中ROS、MDA的含量以及PD-1、PD-L1、TWEAK、Caspase-3、Caspase-9的mRNA表达量均显著高于Sham组,血清中cTnⅠ、CK-MB、SP-A、CC16、KL-6的含量均显著高于Sham组;心肌组织中ROS的含量与血清中cTnⅠ、CK-MB的含量以及心肌组织中PD-1、PD-L1、TWEAK、Caspase-3、Caspase-9的mRNA表达量呈正相关,肺组织中ROS的含量与血清中SP-A、CC16、KL-6的含量以及肺组织中PD-1、PD-L1、TWEAK、Caspase-3、Caspase-9的mRNA表达量呈正相关.结论:脓毒症大鼠模型心肌组织和肺组织中ROS生成增多能够引起组织损伤以及细胞凋亡.%Objective:To study the correlation of reactive oxygen species (ROS) contents in myocardial tissue and lung tissue with tissue injury and apoptosis in the rat models with sepsis.Methods:The SD male rats were selected as experimental animals and divided into two groups:CLIP group were established into sepsis models by cecal ligation and puncture and Sham group received Sham operation.After model establishment,the serum was collected to determine the contents of myocardial injury and lung injury markers,and the lung tissue and myocardial tissue were collected to determine the contents of ROS and MDA as well as the expression of apoptosis genes.Results:ROS and MDA contents as well as PD-1,PD-L1,TWEAK,Caspase-3 and Caspase-9 mRNA expression in myocardial tissue and lung tissue of CLIP group were significantly higher than those of Sham group,and serum cTnⅠ,CK-MB,SP-A,CC16 and KL-6 contents were significantly higher than those of Sham group (P＜0.05);serum ROS content in myocardial tissue was positively correlated with cTnⅠ and CK-MB contents in serum as well as PD-1,PD-L1,TWEAK,Caspase-3 and Caspase-9 mRNA expression in myocardial tissue,and ROS content in lung tissue was positively correlated with SP-A,CC16 and KL-6 contents in serum as well as PD-1,PD-L1,TWEAK,Caspase-3 and Caspase-9 mRNA expression in lung tissue.Conclusion:The increased generation of ROS in myocardial tissue and lung tissue of rat models with sepsis can cause tissue damage and apoptosis.