ICP胎盘中SOCS3表达量与母体Th细胞失衡、胎盘滋养细胞损伤的相关性

摘要

目的:研究妊娠期肝内胆汁淤积症(ICP)胎盘中细胞因子信号传导负调控因子3(SOCS3)表达量与母体T h细胞失衡、胎盘滋养细胞损伤的相关性.方法:选择巴中市中医院2014年6月~2017年8月诊断为ICP的初产妇作为研究的ICP组,另取同期分娩的健康初产妇作为对照组.分娩后取胎盘并测定SOCS3、Th细胞因子、凋亡基因的表达量.结果:ICP组胎盘中SOCS3的mRNA表达量、蛋白表达量以及白介素(IL)-4、IL-13的蛋白表达量均显著低于对照组,γ-干扰素(IFN-γ)、肿瘤坏死因子-α (TNF-α)、IL-2、p53、Caspase-3、Fas、Caspase-8、Cyt-C、Caspase-9的蛋白表达量显著高于对照组;SOCS3低表达的ICP胎盘中IFN-γ、TNF-α、IL-2、p53、Caspase-3、Fas、Caspase-8、Cyt-C、Caspase-9的蛋白表达量显著高于SOCS3高表达的ICP胎盘,IL-4、IL-13的蛋白表达量显著低于SOCS3高表达的ICP胎盘.差异均有统计学意义(P<0.05).结论:ICP胎盘中SOCS3的低表达能够加重 Th细胞失衡及凋亡所致胎盘滋养细胞损伤.%Objective:To study the correlation of suppressor of cytokine signaling 3(SOCS3)expression in placenta of intrahepatic cholestasis of pregnancy(ICP)with maternal Th cell imbalance and placental trophoblast cell damage.Methods:Primiparae who were diagnosed with ICP in Bazhong Hospital of Traditional Chinese Medicine between June 2014 and August 2017 were selected as the ICP group,and the healthy primiparae who gave birth during the same period were selected as the control group.The placenta was collected after delivery to determine the expression of SOCS 3,Th cytokines and apoptosis genes.Results:SOCS3 mRNA expression and protein expression as well as IL-4 and IL-13 protein expression in placenta of ICP group were significantly lower than those of control group(P<0.05);IFN-γ,TNF-α,IL-2,p53,Caspase-3,Fas,Caspase-8, Cyt-C and Caspase-9 protein expression were significantly higher than those of control group(P<0.05);IFN-γ,TNF-α,IL-2, p53,Caspase-3,Fas,Caspase-8,Cyt-C and Caspase-9 protein expression in ICP placenta with lower SOCS3 expression were significantly higher than those in ICP placenta with higher SOCS3 expression whereas IL-4 and IL-13 protein expression were significantly lower than those in ICP placenta with higher SOCS3 expression(P< 0.05).Conclusion:The low expression of SOCS3 in the ICP placenta can aggravate the imbalance of Th cells and the damage of placental trophoblast cells induced by ap-optosis.