癫痫患儿血清及脑脊液中hs-CRP、S100B、NSE含量变化及其与神经细胞凋亡的相关性研究

摘要

Objective:To study the changes of hs-CRP,S100B and NSE levels in serum and cerebrospinal fluid of chil-dren with epilepsy and their correlation with the nerve apoptosis.Methods:The children who were diagnosed with epilepsy in the Hospital between March 2015 and February 2017 were selected as epilepsy group,and the children who underwent opera-tion due to hernia during the same period were selected as control group.The cerebrospinal fluid was collected to determine the contents of hs-CRP,S100B and NSE,and the serum was collected to detect the contents of hs-CRP,S100B,NSE,apoptosis molecules and Sirtuins family molecules.Results:hs-CRP,S100B and NSE levels in serum and cerebrospinal fluid of epilepsy group were significantly higher than those of control group,Bim,Bax,Caspase-3,Caspase-4 and Caspase-9 levels in cerebro-spinal fluid were significantly higher than those of control group,and XIAP,Bcl-2,SIRT1,SIRT2,SIRT3,SIRT4,SIRT5, SIRT6 and SIRT7 levels in cerebrospinal fluid were significantly lower than those of control group;hs-CRP,S100B and NSE levels in serum and cerebrospinal fluid of children with epilepsy were positively correlated with Bim,Bax,Caspase-3,Caspase-4 and Caspase-9 levels in cerebrospinal fluid,and negatively correlated with XIAP,Bcl-2,SIRT1,SIRT2,SIRT3,SIRT4, SIRT5,SIRT6 and SIRT7 levels in cerebrospinal fluid.Conclusion:The abnormally elevated hs-CRP,S100B and NSE in serum and cerebrospinal fluid of children with epilepsy are closely related to the excessive apoptosis of nerve cells.%目的:癫痫患儿血清及脑脊液中hs-CRP、S100B、NSE含量变化及其与神经细胞凋亡的相关性.方法:选择在本院诊断为癫痫的患儿作为癫痫组,同期因疝气接受手术的患儿作为对照组.采集脑脊液并测定hs-CRP、S100B、NSE的含量,采集血清并检测hs-CRP、S100B、NSE及细胞凋亡分子、Sirtuins家族分子的含量.结果:癫痫组患儿血清及脑脊液中hs-CRP、S100B、NSE的含量显著高于对照组,脑脊液中Bim、Bax、Caspase-3、Caspase-4、Caspase-9的含量显著高于对照组,脑脊液中 XIAP、Bcl-2、SIRT1、SIRT2、SIRT3、SIRT4、SIRT5、SIRT6、SIRT7的含量显著低于对照组;癫痫患儿血清及脑脊液中hs-CRP、S100B、NSE的含量与脑脊液中Bim、Bax、Caspase-3、Caspase-4、Caspase-9的含量呈正相关,与脑脊液中XIAP、Bcl-2、SIRT1、SIRT2、SIRT3、SIRT4、SIRT5、SIRT6、SIRT7的含量呈负相关.结论:癫痫患儿血清及脑脊液中异常升高的hs-CRP、S100B、NSE与神经细胞过度凋亡密切相关.