结肠癌组织中SphK1和FAK表达量与临床病理特征、上皮间质转化的相关性研究

摘要

Objective:To investigate the correlation of SphK1 and FAK expression in colon cancer tissue with clinical pathological features and epithelial-mesenchymal transition (EMT).Methods:90 patients with colon cancer who received radical operation for colon cancer in our Hospital between January 2015 and May 2017 were selected as colon cancer group,and 48 patients with intestinal perforation who received emergency surgery in this hospital during the same period were selected as control group.The differences in the expression of SphK1,FAK as well as proliferation,invasion and EMT marker genes in lesion tissue were compared between the two groups.Pearson test was used to evaluate the correlation of SphK1 and FAK gene expression with the malignant biological characteristics of tumor and EMT in colon cancer tissues.Results:SphK1 and FAK mRNA expression in lesion tissue of colon cancer group were higher than those of control group;proliferation-related genes Mina53,GTPBP4,CXCR7 and EZH2 mRNA expression were higher than those of control group whereas MS4A12 mRNA expression was lower than that of control group;invasion-related genes Lin28A,PRDX1,PRM1 and SLP-2 mRNA expression were higher than those of control group whereas ZNRD1 mRNA expression was lower than that of control group;EMT marker gene E-cadherin mRNA expression was lower than that of control group whereas N-cadherin and vimentin mRNA expression were higher than those of control group.Pearson test showed that the SphK1 and FAK gene expression in colon cancer tissue were positively correlated with the tumor proliferation and invasion activity and the EMT process.Conclusion:SphK1 and FAK gene expression are abnormal in colon cancer tissue and can increase the proliferation and invasion activity of tumor cells and accelerate the EMT process.%目的:探讨结肠癌组织中SphK1和FAK表达量与临床病理特征、上皮间质转化(EMT)的相关性.方法:2015年1月～2017年5月间在我院接受结肠癌根治术治疗的结肠癌患者90例作为结肠癌组,同期在本院进行急诊手术治疗的肠穿孔患者48例作为对照组.对比两组病灶组织中SphK1、FAK基因,增殖、侵袭、EMT标记基因的表达量差异.采用Pearson检验评估结肠癌组织中SphK1、FAK基因表达量与肿瘤恶性生物学特征、EMT的相关关系.结果:结肠癌组病灶组织中SphK1、FAK mRNA表达量高于对照组(P＜0.05);增殖相关基因Mina53、GTPBP4、CXCR7、EZH2 mRNA的表达量高于对照组(P＜0.05),MS4A12 mRNA的表达量低于对照组(P＜0.05);侵袭相关基因Lin28A、PRDX1、PRM1、SLP-2 mRNA的表达量高于对照组(P＜0.05),ZNRD1 mRNA的表达量低于对照组(P＜0.05);EMT标记基因E-cadherin mRNA的表达量低于对照组(P＜0.05),N-cadherin、vimentin mRNA的表达量高于对照组(P＜0.05).Pearson检验发现,结肠癌组织中SphK1、FAK基因表达量与肿瘤增殖侵袭活性、EMT进程呈正相关.结论:结肠癌组织中存在SphK1、FAK基因异常表达量,可促进肿瘤细胞增殖、侵袭活性增加,加速EMT进程.