马兰的薄层色谱研究

摘要

Objective： To establish a TLC method for quality of Kalimeris indica （L.） Sch-Bip. Method： α-spinasterl-3-O-β-D-glucoside was identified in TLC chromategram by using the mixed solvent chloroform-methanol （9：1 ） （ volume ratio） as developing solvent after being extracted with different solvents of Kalimeris indica （L.）Sch-Bip. Results： The spots was clear and the separation was good with the simplemethod. Conclusion：The method was found to be rapid and effective.%目的：建立马兰药材的薄层色谱鉴别方法。方法：马兰药材以不同溶剂经超声提取后,以氯仿：甲醇=9∶1（体积比）为展开系统,对马兰药材中α-菠甾醇-3-O-β-D-葡萄糖苷作定性鉴别。结果：采用此法检测,斑点清晰,分离效果好。结论：建立了一种快速、有效的马兰药材的薄层色谱鉴别方法。