金铁锁总皂苷含量测定

摘要

To establish the determination methods of total saponins from Psammosilene tunicoides. Methods： The oleanolic acid was selected as comparison product and 5% vanillin-acetic acid-HCLO solution was selected as color-producing reagent. Determiningn absorbability in the point of 540nm by spectrophotometry method. And calculating the total saponins in Psammosilene tunicoides. Results： The calibration curve was linear over the range of 1 ~ 101~g. mL-I with the correlation of 0. 998 4, the average recoveries of dioscin was 97.9% （n = 6 ）, RSD = 1.61% ; the highest content of total saponins is Sichuan, Yunnan, followed by Guizhou minimum. Conclusion： Psammosilene tunicoides of ultrasonic extraction of total saponin assay method is simple, accurate, good stability. It can be used for quality control of medicinal Psammosilene tunicoides.%建立金铁锁总皂苷的含量测定方法。方法：以齐墩果酸为对照品,5%香草醛冰醋酸、高氯酸为显色剂,采用分光光度法,在540nm处测定吸光度,计算金铁锁中总皂苷的含量。结果：金铁锁总质量浓度在1～10μg.mL-1与吸光度呈良好的线性关系（r=0.998 4）,平均回收率为97.9%（n=6）,RSD=1.61%;金铁锁中总皂苷含量四川最高,云南次之,贵州最低。结论：建立了金铁锁中总皂苷超声提取含量测定方法,此方法简便、准确,稳定性好、可用于金铁锁药材的质量控制。