Objective To establish an ultraviolet spectrophotometric method for the assay of the total flavonoids from Herba Pogostemonis. Methods After being extracted at the temperature of 251 by methanol, the samples were purified through polyamide column chromatography, and then the assay of the total flavonoids was performed with two specific methods. The two methods were ultraviolet spectrophotometric method with apigenin as reference substance, and visible spectrophotometric method with Rutin as reference substance and with sodium nitrite and aluminum nitrate as the complex color developing reagent. Results With apigenin as the reference substance, the content of the total obtained flavonoids was 0. 83 mg/g in the samples from Zhanjiang, Guangdong, 0.77 mg/g from Zhixin Company, 0. 85 mg/g from Guangzhou, Guangdong, 0. 94 mg/g from Hanoi, Vietnam, and 0. 97 mg/ g from Viet Tri, Vietnam. With rutin as the reference substance in visible spectrophotometric method, the content of the total obtained flavonoids was 0. 81 mg/g in the samples from Zhanjiang, Guangdong, 0. 74 mg/g from Zhixin pharmaceuticat Company, 0.69 mg/g from Guangzhou, Guangdong, 0. 73 mg/g from Hanoi, Vietnam, and 0. 71 mg/g from Viet Tri, Vietnam. By using apigenin as the reference substance, the content of the gained total flavonoids was higher than that by using rutin as the reference substance. Conclusion For the assay of total flavonoids, ultraviolet spectrophotometry is more stable than visible spectrophotometry, and is faster and easier.%[目的]建立广藿香中总黄酮含量的测定方法.[方法]各样品经甲醇提取后,在25℃温度下经聚酰胺柱层析纯化后,分别采用芹菜素—紫外分光光度法和芦丁—亚硝酸钠加硝酸铝络合法于可见光下测定总黄酮含量.[结果]以芹菜素为参照物测得含量的结果为:中国湛江0.83 mg/g、致信药业0.77 mg/g、广东广州0.85 mg/g、越南河内0.94 mg/g、越南河池0.97 mg/g;以芦丁为参照物测得结果为:中国湛江0.81 mg/g,致信药业0.74 mg/g、广东广州0.69 mg/g、越南河内0.73 mg/g、越南河池0.71 mg/g.以芹菜素为参照物测得量高于以芦丁为参照物测得量.[结论]可见分光光度法用于广藿香中总黄酮含量的测定不稳定,紫外分光光度直接测定法则稳定,且快速、简便.
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