补肾活血方对骨关节炎软骨细胞低氧诱导因子-1αmRNA的影响

摘要

Objective To study the influence of Bushen Huoxue Recipe (BHR) on hypoxia inducible factor (HIF-lα) mRNA expression in chandrocytes of osteoarthritis (OA) rats. Methods Thirty-two SD rats were randomly divided into 4 groups: normal group, model group, BHR group, and celecoxib group. The OA models were established by using Hulth method. The sections were stained with Safranin O for histological examination under light microscope. The cartilaginous histological characteristics of each sample were evaluated according to the scoring method of Mankin. Real-time fluorescent quantitative PCR was used to detect the expression of HIF-lα mRNA in each group. Results The results of Safranin O staining showed that Mankin score was increased in the model group (P ＜ O. 05 compared with that in the normal group). Mankin score was decreased or undetectable in BHR group (P ＜0. 05 compared with that in celecoxib group and the model group). HIF-lα mRNA expression was increased in the model group (P ＜0. 01 compared with that in the normal group), and was decreased in BHR group (P ＜0. 05 compared with that in celecoxib group and the model group). Conclusion BHR can delay the development of OA probably by inhibiting the up-regulation of HIF-Iα mRNA expression.%[目的]观察补肾活血方对骨关节炎软骨细胞中低氧诱导因子(HIF)-1α mRNA表达的影响.[方法]32只SD大鼠随机分为正常组、模型组、中药组、塞来昔布组(西药组),每组8只,按照Hulth法复制膝骨关节炎模型,取关节软骨,对关节软骨病理切片采用Safranin O染色,光镜下观察,进行Mankin评分,并采用实时荧光定量聚合酶链反应检测各组HIF-1α mRNA的表达.[结果]模型组Safranin O染色的Mankin评分较正常组显著升高(P＜0.05);中药组Safranin O染色减少或缺失程度较西药组、模型组轻,差异有显著性意义(均P＜0.05).模型组HIF-1αmRNA 表达较正常组升高,差异有显著性意义(P＜0.01);中药组HIF-1αmRNA表达低于西药组、模型组,差异有显著性意义(均P＜0.05).[结论]补肾活血方可能通过抑制HIF-lαmRNA上调,从而延缓骨关节炎的发展.