毛橘红总黄酮对酒精性肝损伤大鼠炎性细胞因子的影响

摘要

Objective To study the blood-activating and stasis-removing action of Compound Fengshining Injection (CFI). Methods Rats and mice models of acute blood stasis were induced by adrenaline and ice bath. The effects of CFI on blood hemorheology in rats and on auricular microcirculation in mice were observed. Results CFI relieved the blood-stasis symptoms, and decreased whole blood viscosity, plasma viscosity and hematocrit in model rats (P ＜0. 05 or P ＜0. 01 compared with those in the model group). CFI also promoted auricular blood flow in blood-stasis mice ( P ＜ 0.05 compared with those in the model group). Conclusion CFI shows certain actions of activating blood and removing blood-stasis, and can improve the microcirculation.%[目的]探讨毛橘红总黄酮提取物对酒精性肝损伤的保护作用.[方法]采用二锅头灌胃4周,结合喂养高脂饲料复制慢性酒精性肝损伤大鼠模型,造模同时分别给予不同浓度毛橘红总黄酮提取物,分别检测各组动物血清谷丙转氨酶(AST)、谷草转氨酶(ALT)含量,采用酶联免疫吸附(ELISA)法检测各组大鼠血清中肿瘤坏死因子-α(TNF-α)、转化生长因子β1(TGF-β1)和白细胞介素6(IL-6)的含量.[结果]与空白对照组比较,模型组ALT及AST含量显著升高(P＜0.01),与模型组比较,不同剂量毛橘红总黄酮(217mg/kg、433mg/kg、108mg/kg)均可不同程度降低ALT、AST、TNF-α、TGF-β1和IL-6的含量.[结论]毛橘红总黄酮可降低炎性细胞因子TNF-α、TGF-β1和IL-6的含量,对慢性酒精性肝损伤具有一定的保护作用.