高效液相色谱法测定骨康口服液中芍药苷的含量

摘要

Objective To establish a quality control method for paeoniflorin in Gukang oral liquid.Methods High performance liquid chromatography (HPLC) was performed on BDS Hypersil C18 (4.6 mm× 250 mm,5 μm),the mobile phase consisted of acetonitrile-0.1％ phosphoric acid solution (10 ∶ 90) at a flow rate of 1.0 mL/min,and the detection wavelength was 230 nm.Results The linear range of paeoniflorin was from 30.36 to 121.42 μg·mL-1 (r=0.999 8).The average recovery of paeoniflorin was 98.2％ with RSD being 2.0％ (N=9).Conclusion The established method is simple,efficient and accurate,and can be used for the quality control of paeoniflorin in Gukang oral liquid.%[目的]建立骨康口服液中芍药苷质量控制方法.[方法]采用高效液相色谱(HPLC)法测定芍药苷的含量,以BDSHypersil C18(4.6 mm×250 mm,5μm)为色谱柱,流动相为乙腈—体积分数0.1％磷酸溶液(10∶90),流速1.0 mL/min,检测波长230 nm.[结果]芍药苷在30.36～121.42 μg/mL呈良好的线性关系(r=0.999 8),回收率为98.2％ (sR 2.0％,N=9).[结论]本法简便,准确,灵敏度高,重现性好,可用于骨康口服液的控制.