基于肝功能评价不同游泳训练周期复制运动性疲劳模型的研究

摘要

Objective To study the influence of swimming training for different periods on rat liver function, and to evaluate the quality of exercise-induced fatigue rat model established by swimming training for different periods. Methods Forty SD rats were randomly divided into 5 groups, namely blank group, and 7-, 14-, 21-and 28-day exercise groups, 8 rats in each group. Rats in the blank group were fed with normal diet, but were not given exercise training. The other groups were given exercise training for 7, 14, 21 and 28 days respectively, two times a day, 15 minutes every time and with 10-minute resting interval between the two times. At the last day of the experiment, all rats were executed by neck dislocation for the sampling of blood and liver tissues, and then the serum contents of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) were determined. And the contents of total anti-oxidization capacity (T-AOC), malondialdehyde (MDA), lactic acid (LA), lactate dehydrogenase (LDH) and glucose (Glu) in liver tissues were also examined. Results Compared with the blank group, the contents of ALT and AST were increased in 7-, 14-, 21-, and 28-day exercise groups, especially in 21-and 28-day exercise groups (P<0.01) . The contents of T-AOC and MDA were increased in 7-, 14-, 21-, and 28-day exercise groups (P<0.01), the effects on T-AOC in the 21-and 28-day exercise groups and on MDA in the 28-day exercise group being more obvious (P<0.05 or P<0.01) . The LA content and LDH activity were increased and the glycogen was decreased in the liver tissues of 7-, 14-, 21-, and 28-day exercise groups, and the increase of LA content in 21- and 28-day exercise groups and that of LDH activity in 28-day exercise groups were more significant ( P<0.05 or P<0.01). The hepatic glycogen content in 14-, 21-, and 28-day exercise groups were lower than that in the other groups (P<0.01). Conclusion With hepatic function injury in exercise-induced fatigue as the criteria, the exercise-induced fatigue rat model has been successfully established in this study, and the model established by swimming training for 21 and 28 days is more steady.%目的研究不同游泳训练周期对大鼠肝功能的影响，从肝功能角度评价采用不同游泳训练周期复制运动性疲劳大鼠模型的效果。方法选用40只SD大鼠随机均分为空白组、运动7 d组、运动14 d组、运动21 d组和运动28 d组共5组，每组8只，空白组大鼠给予正常饮食，不参与运动训练，其他各组大鼠分别进行游泳训练7、14、21、28 d，每天训练2次，每次15 min，间隙10 min。在实验最后1天断头取血，检测血清谷丙转氨酶（ALT）和谷草转氨酶（AST）含量；取同部位适量肝组织，测定肝总抗氧化能力（T－AOC）活性、肝丙二醛（MDA）含量、肝乳酸（LA）含量、肝乳酸脱氢酶（LDH）活性和肝葡萄糖（Glu）含量。结果与空白组比较，游泳训练7、14、21、28 d组血清ALT和AST含量均显著升高，尤其是运动21、28 d组升高更显著（P＜0．01）；游泳训练7、14、21、28 d组肝T－AOC活性和MDA含量均显著升高（P＜0．01），其中游泳训练21、28 d组T－AOC活性升高更显著，而28 d组MDA含量升高最显著（P＜0．05或P＜0．01）；游泳训练7、14、21、28 d组，肝LA含量和LDH活性均显著增加，而肝糖原含量均显著降低，其中游泳训练21、28 d组肝LA含量升高更显著，游泳运动28 d组LDH活性增加最显著（P＜0．05或P＜0．01），游泳训练14、21、28 d组肝糖原含量降低更显著（P＜0．01）。结论以运动性疲劳状态下肝功能损伤为评价标准，本研究造模方法可成功复制运动性疲劳大鼠模型，但造模效果以21 d和28 d更为稳定。