不同产地鸡血藤药材高效液相指纹图谱及芒柄花素含量分析

摘要

Objective To establish the fingerprints and formononetin content determination method for Caulis Spatholobi from different habitats by high performance liquid chromatography ( HPLC) , thus to control the quality of Caulis Spatholobi. Methods Reversed phase-high performance liquid chromatography (RP-HPLC) for fingerprint was performed on Feini Gen RedClassical AQ-C18 column ( 4.6 mm × 250 mm, 5 μm) with acetonitrile-0.1%acetic acid solution as the mobile phase by gradient elution, and the detection wavelength was 260 nm. High performance liquid chromatography-diode array detector ( HPLC-DAD) for the determination of formononetin content was performed on AcclaimTM 120-C18 column ( 4.6 mm × 250 mm, 5 μm) with acetonitrile-water solution by isocratic elution, the detection wavelength was 254 nm, the flow rate was 1.0 mL/min and the column temperature was 25℃. Results The standard fingerprint of Caulis Spatholobi was set up through the evaluation of the fingerprints of 24 batches of Caulis Spatholobi samples from different habitats. Thirteen common peaks were identified with reference to formononetin peak, and the content of formononetin was determined by HPLC-DAD method. The similarity of the fingerprints of Caulis Spatholobi from different habitats and their formononetin content had great differences. Conclusion The established method is simple, accurate, highly sensitive, and repeatable, and can be applied for the quality control of Caulis Spatholobi.%【目的】建立鸡血藤药材的高效液相（HPLC）指纹图谱和芒柄花素含量测定的方法，评价不同产地鸡血藤的质量。【方法】采用反相高效液相色谱法（RP-HPLC）建立鸡血藤指纹图谱，以Feini Gen Red Classical AQ-C18(4.6 mm ×250 mm，5μm)为色谱柱，乙腈—体积分数0.1%冰醋酸溶液进行梯度洗脱，检测波长260 nm；芒柄花素含量测定以AcclaimTM 120-C18柱(4.6 mm ×250 mm，5μm)为色谱柱，乙腈—水等度洗脱，检测波长为254 nm，流速1.0 mL/min，柱温25℃。【结果】对24批来自不同产地的鸡血藤色谱指纹图谱进行评价，建立了鸡血藤标准指纹图谱，以芒柄花素为参照峰，确定了13个共有峰，并应用高效液相色谱—二极管阵列检测器（HPLC-DAD）法测定样品中芒柄花素的含量。不同产地鸡血藤药材指纹图谱相似度和芒柄花素含量差异较大。【结论】本研究所建立的方法简便准确、灵敏度高、重复性好，可作为鸡血藤的药材质量控制方法。