Objective To investigate the effect of Zhali Nusi Recipe ( ZNR) on hemorheological parameters, blood - lipid metabolism, the activities of superoxide dismutase ( SOD) and glutathione peroxidase ( GSH-Px) , and the content of malondialdehyde (MDA) in brain tissues of cerebral ischemia-reperfusion rats with the syndrome of phlegm blended with blood stasis. Methods Ninety rats were randomly divided into sham operation group, model group, nimodiping (10.8 mg/kg) group, high-, middle- and low-dose ZNR groups ( in the dosage of 30, 15, 7.5 g/kg, respectively) , 15 rats in each group. Except the sham operation group, the other groups were given intragastric administration of intralipid for 4 weeks, and then were induced into focal cerebral ischemia-reperfusion by middle cerebral artery occlusion with thread-obstruction method. After post-operation medication for 7 days, the rat general situations, the neurological deficiency symptoms, and the water content in brain tissues were observed. The morphological features of brain tissues were observed after HE staining. The blood was taken from the ab dominal aorta to detect the hemorheological parameters and blood lipid levels. Metabolic levels of free radicals in the brain tissue homogenate were also determined. Results Compared with the sham operation group, neurological deficiency scores of the rats and the water content in the brain tissues of model group were significantly enhanced. The hippocampal neurons of model rats presented typical ischemic changes. In the model group, the hemorheological parameters such as whole blood viscosity, plasma viscosity, hematocrit ( HCT) , and erythrocyte aggregation in dexes ( EAI) were significantly increased, and erythrocyte deformation indexes were significantly decreased; the serum levels of total cholesterol ( TC) , triglyceride ( TG) , low-density lipoprotein cholesterol (LDL-C) were significantly increased, and high-density lipoprotein cholesterol ( HDL-C) level was significantly decreased; the activities of SOD and GSH-Px in brain tissues were significantly decreased, and MDA content was increased significantly ( P<0.05 or P<0.01). Compared with the model group, high-, middle-and low-dose ZNR improved the general situations of the cerebral ischemia-reperfusion rats, increased the neurological deficiency scores, reduced the water content in brain tissues, improved the recovery of morphology of cells in cerebral ischemia tissues, reduced whole blood viscosity, plasma viscosity, HCT and EAI of the model rats, promoted erythrocyte deformation indexes, decreased serum TG, TC, LDL-C levels, increased the activities of SOD and GSH-Px in cerebral ischemic tissues, and sig nificantly decreased MDA content (P<0.05 or P<0.01). And high-dose ZNR had the best effect. Conclusion ZNR has certain protective effect on cerebral ischemia-reperfusion rats with the syndrome of phlegm blended with blood stasis, and the mechanism may be closely related with the improvement ofhemorheology, blood lipid and free radical metabolism in model rats.%【目的】探讨扎里奴思方对痰瘀互结型脑缺血再灌注大鼠血液流变学、血脂代谢的改善作用，以及对脑组织中超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)活性及丙二醛(MDA)含量的影响。【方法】将90只大鼠随机分为假手术组，模型组，尼莫地平组（剂量为10.8 mg/kg），扎里奴思方高、中、低剂量组(剂量分别为30、15、7.5 g/kg)，每组15只；除假手术组外，其余各组均给予脂肪乳剂灌胃4周后，采用栓线法复制局灶性脑缺血(MCAO)再灌注模型。术后给药7 d，观察各组大鼠的一般情况、神经缺失症状、脑组织含水量，采用苏木素—伊红（HE）染色法观察脑组织病理学形态，腹主动脉取血进行血液流变学、血脂的检测，脑组织匀浆测定自由基代谢的水平。【结果】与假手术组比较，模型组大鼠神经功能评分显著升高，脑组织含水量显著增加，大鼠海马神经元出现典型缺血损伤表现，血液流变学中全血黏度、血浆黏度、红细胞压积、红细胞聚集指数显著升高，红细胞变形指数显著下降，血清中总胆固醇(TG)、甘油三脂(TC)、低密度脂蛋白(LDL-C)水平显著升高，高密度脂蛋白胆固醇(HDL-C)显著降低，脑组织中SOD、 GSH-Px活性显著降低， MDA含量显著升高（均P＜0.05或P＜0.01）；与模型组比较，扎里奴思方各剂量组均能改善痰瘀互结型脑缺血再灌注大鼠一般情况，改善神经功能学评分，减轻脑组织含水量，促进脑缺血组织细胞形态恢复；并能降低模型大鼠全血黏度、血浆黏度、红细胞压积、红细胞聚集性，提高红细胞变形性，降低血清TG、 TC、 LDL-C水平，提高缺血脑组织SOD、 GSH-Px活性，降低MDA含量（均P＜0.05或P＜0.01），尤以扎里奴思方高剂量组作用显著。【结论】扎里奴思方对痰瘀互结型脑缺血再灌注大鼠损伤具有良好的保护作用，其机制可能与改善模型大鼠血液流变学、血脂和自由基代谢密切相关。
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