超滤陶瓷膜精制黄芪葛根汤组成药物提取液的工艺参数研究

摘要

[目的]优化无机陶瓷膜精制黄芪葛根汤方剂中黄芪、 葛根药材提取液的工艺参数.[方法]以黄芪和葛根(剂量配比为1:2)的提取液为研究对象,以含固量得率、 葛根素转移率及特征峰面积之和的减少率为指标,通过对膜孔径、 料液温度和超滤压力等3个因素进行单因素与正交试验优化.[结果]以综合得分为评价指标,最佳优化参数如下:膜孔径为100 nm,料液温度为35℃,超滤压力为0.18 MPa.陶瓷膜孔径和超滤压力为主要影响因素,对精制工艺有显著性影响(P<0.05).[结论]采用无机陶瓷膜超滤技术精制黄芪葛根汤方提取液是可行的,为陶瓷膜在中药提取液的精制研究方面提供了可靠的实验依据.%Objective To optimize the process parameters for the purification of the extract solution of Radix Astragali and Radix Puerariae in Huangqi Gegen Decoction prescription by ceramic membrane ultrafiltration. Methods With the extract solution of Radix Astragali and Radix Puerariae (in the proportion of 1 : 2) as the study object, and with solid content, puerarin transfer rate and characteristic peak area as indicators, the membrane pore size, ultrafiltrate temperature and ultrafiltration pressure were selected as the main factors, and single factor test and L9 (34) orthogonal test were used to optimize purification conditions. Results With comprehensive scores as the evaluation index, the optimized conditions for the extract solution were as follows:membrane pore size was 100 nm, ultrafiltration temperature was 35 ℃, and ultrafiltration pressure was 0.18 MPa. The main influence factors of ceramic membrane pore size and ultrafiltration pressure had obvious effects on the refined process(P<0.05).Conclusion The method of purification of the extract solution of Huangqi Gegen Decoction prescription by ceramic membrane ultrafiltration is feasible , and the results will provide reliable experimental evidence for the purification of Chinese herbal medicine extract with ceramic membrane.