'通调针法'电针治疗肝郁型乳腺增生病大鼠的疗效机制r及其与迷走神经调节的关系

摘要

Objective To explore the actions of vagus nerve in electroacupuncture (EA) with unblocking and regulating needling therapy for the treatment of mammary gland hyperplasia(MGH)with liver stagnation type, and to provide experimental evidences for clinical usage of EA with unblocking and regulating needling therapy. Methods Sixty healthy SD rats were randomly divided into 5 groups,namely blank control group,model control group,EA group,EA with disconnection of vagus nerve group,and sham-operation EA group,12 rats in each group. Except for the blank control group, the rats in the remaining groups were modeled. After successful establishment of the model of mammary gland hyperplasia (MGH) with liver stagnation type, the rats in EA group with disconnection of vagus nerve were given disconnection of unilateral vagotomy operation, the rats in sham-operation EA group were given sham operation, and EA group had no treatment, and then all of the 3 treatment groups were given EA with unblocking and regulating needling therapy. At the end of the experiment, the diameter and height of rat nipple were measured,the serum contents of estradiol (E2)and progestin (P)in the rats were detected by enzyme-linked immunoassay(ELISA),and the contents of anti-estrogen receptor alpha (ERα)and anti-progesterone receptor(PR)in the rat breast tissue were detected by immunofluorescence double staining and Western blotting method. Results (1)EA treatment can obviously improve the diameter and height of MGH rat nipple, but the therapeutic effect of EA group with disconnection of vagus nerve was not obvious. (2)Compared with the model control group,the levels of serum E2 and mammary ERαwere markedly reduced(P<0.01),and serum P level and mammary PR content were obviously increased in EA group and sham-operation EA group (P < 0.01). However,the above indexes had no obvious changes in EA group with disconnection of vagus nerve (P>0.05). Conclusion The therapeutic mechanism of EA with unblocking and regulating needling therapy for liver-stagnation MGH may be closely related with the regulation of vagus nerve.%[目的]探讨迷走神经在"通调针法"电针治疗肝郁型乳腺增生病(MGH)中的作用,为"通调针法"电针的临床应用提供实验依据.[方法]将60只SD大鼠随机分为空白对照组、模型对照组、电针治疗组、切断迷走神经电针治疗组、假手术电针治疗组,共5组,每组12只.除空白对照组外,其余大鼠均复制成肝郁型MGH病证结合模型.造模成功后,切断迷走神经电针治疗组实施单侧迷走神经切断术,假手术电针治疗组实施假手术,电针治疗组不做任何干预,在此基础上,3个治疗组均给予"通调针法"电针治疗.实验结束时,测量各组大鼠乳头高度、直径;采用酶联免疫吸附(ELISA)法检测大鼠血清中雌二醇(E2)、孕激素(P)表达水平;采用免疫荧光双染法及免疫印迹(Western blot)法检测大鼠乳腺组织中雌二醇α受体(ERα)、孕激素受体(PR)含量.[结果](1)电针治疗可明显改善MGH大鼠乳头直径、高度,而切断迷走神经电针治疗组改善不明显.(2)与模型对照组比较,电针治疗组、假手术电针治疗组大鼠血清E2水平和乳腺组织ERα含量显著降低(P<0.01),血清P水平和乳腺组织PR含量显著升高(P<0.01);而切断迷走神经电针治疗组上述指标无明显变化(P>0.05).[结论]"通调针法"电针治疗肝郁型MGH的疗效机制可能与迷走神经调节的关系密切.