黄粉虫纤溶活性蛋白免疫原性与抗血栓作用的研究

摘要

为了研究黄粉虫纤溶活性蛋白（ Tenebrio Fibrinolytic Proteins，TFP）的免疫原性及其抗血栓作用，本研究将纯化的TFP经皮下注射免疫小鼠，间接ELISA法检测小鼠血清中的抗体含量及抗体效价；小鼠灌胃TFP后，取血浆检测凝血酶时间（thrombin time，TT）、凝血酶原时间（prothrombin time，PT）、活化部分凝血活酶时间（activated partial thromboplastin time ，APTT）、组织型纤溶酶原激活剂（ tissue-type plasminogen activator ，t-PA）和其抑制物（ plasminogen activator inhibitor ，PAI），角叉菜胶使小鼠尾部形成血栓，测量血栓形成长度。结果显示，TFP免疫小鼠后产生特异性抗体且随着免疫次数的增加，抗体含量逐渐升高，末次免疫后产生的抗体效价为1∶640；TFP可以显著延长TT，增强t-PA的活性，抑制PAI的活性，同时显著抑制由角叉菜胶引起的小鼠尾部血栓的形成。这说明TFP具有良好的免疫原性且具有一定的抗血栓作用。%To study the immuongenicity and anti-thrombotic effect of Tenebrio Fibrinolyric Proteins ( TFP) , mice were immunized with purified TFP by subcutaneous injection , then the antibody content and antibody titer were detected by the optimal ELISA method .Mice were gavaged with purified TFP , then thrombin time ( TT) , prothrombin time ( PT) , activated partial thromboplastin time ( APTT) , tissue-type plasminogen activator ( t-PA) and plasminogen activator inhibitor ( PAI) activity were detected .The results show that the specific antibody is found after TFP immunized mice and the antibody content in -creases when the number of immunization rises .The specific antibody titer is 1∶640 after the last immuni-zation .The TT of mice is remarkably prolonged after using TFP .The t-PA activity increases while the PAI activity decreases .Meanwhile the average thrombosis length of tail is significantly reduced .This shows that the TFP has good immunogenicity and anti-thrombotic effect .