3，5-二溴水杨醛缩甘氨酸的合成及荧光法测定粮食中微量锌

摘要

合成了新型席夫碱试剂3，5-二溴水杨醛缩甘氨酸（DBSAGLY），建立该试剂荧光度法测定微量Zn（Ⅱ）的新体系，在pH＝7.0～8.0时Zn(Ⅱ)与DBSAGLY形成1∶1的络合物，络合物最大激发和发射波长分别为λex＝300 nm和λem＝468 nm，荧光线性范围0～120 μg/L，检测限量1.6 μg/L。本法用于粮食中锌的测定。%A new shciff's base 3,5-dibromsalicyladehyde-glycocoll(DBSAGLY) was synthezied and its characteristics were determined. The optimal conditions for the fluorescent complex formation of DBSAGLY with Zinc(Ⅱ) were established. The maxinum excitation and emission wavelength is 300 nm and 468 nm respectively at pH 7.0～8.0. The molar ratio of Zinc to DBSAGLY was determined to be 1∶1. The proposed method was used for the determination of Zinc in crops in the range of 0～120 μg/L. The detection limit of Zn(Ⅱ) is 1.6 μg/L.