Objective To measure and compare routine blood indexes in the different rat models with syndrome of spleen qi deficiency.Methods Based on the method of restricted diet in rats,three rat models with spleen qi deficiency were established by Folium Sennae induced bitter cold diarrhea,swimming induced over-fatigue and combination of the above two means respectively.Furthermore,the changes of physical sign,thymus and spleen indexes,the serum D-xylose value,and the pathological tissue section of spleen in these models were evaluated,and the routine blood indexes were also measured.Results Compared to the control group,spleen qi deficiency rats showed that WBC and LY# were significantly lower than those in control group (P<0.05) and the most significant changes of all indexes were observed in combination group(P<0.01).And only in combination group,MONO#,NEUT#,HGB,RBC,HCT,MCV,MCH were decreased significantly (P<0.05).Conclusion Combining bitter cold diarrhea and over-fatigue with restricted diet to establish the model is the best suited to the spleen qi deficiency syndrome due to its low immunity and anemia.And WBC、LY#、HCT、MCH could be the potential steady markers as the index to evaluate spleen qi deficiency model.%目的 测定不同造模方法复制脾气虚证模型的血常规指标并进行比较.方法 大鼠在限食法的基础上分别采用番泻叶苦寒泻下、游泳劳倦过度、番泻叶苦寒泻下加游泳劳倦过度3种方法建立脾气虚证模型,根据其表征变化、血清D-木糖检测、胸腺指数和脾脏指数、脾的病理组织切片等多指标综合评价所建的模型,测定血常规指标并进行比较.结果 与对照组比较,模型组的白细胞计数(WBC)、淋巴细胞计数(LY#)均显著降低(P<0.05),其中番泻叶加游泳复合模型的变化最为明显(P<0.01),与其他模型不同的是,复合模型的单核细胞计数(MONO#)、中性粒细胞计数(NEUT#)、红蛋白含量(HGB)、红细胞计数(RBC)、红细胞比容(HCT)、平均红细胞体积(MCV)、红细胞血红蛋白含量(MCH)的变化均具有显著性(P<0.05).结论 以苦寒泻下加劳倦过度加限食法三因素复合的方法建立的脾气虚模型稳定性良好,表现免疫功能下降、贫血等症状符合中医症候,且WBC、LY#、HCT、MCH具有潜能成为评价脾气虚症的稳定性指标.
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