以玻碳电极为基底,在电极表面修饰一层氧化石墨烯-硫堇(GO -Th)薄膜,通过层层自组装方式,将纳米金和葡萄糖氧化酶(GOD)固定在玻碳电极表面,制得一种新型电流型葡萄糖生物传感器.在优化的实验条件下,该生物传感器对葡萄糖的线性响应范围为1.0×10-9 ～5.7×10-5 mol·L-1,检测下限为5.0×10-10mol·L-1.该传感器具有制备方法简单、灵敏度高、稳定性好等特点.%A new amperometric biosensor for the determination of glucose was proposed by using gold nanoparticles and graphene oxide - thionine conjugates as matrices. Thionine molecules were initially adsorbed onto the surface of graphene oxide via π -π binding reaction, then the thionine - graphene conjugations were immobilized on a glassy carbon electrode, which were used for the adsorption of gold nanoparticles, and then glucose oxidase was conjugated onto the gold nanoparticles. Under optimal conditions, the biosensor exhibited a wide linear range of 1.0×10-9 ~5.7×10-5 mol ? L-1 with a low detection limit of 5.2 ×10-10 mol ? L-1 glucose (at S/N = 3). In addition, the stability, repro ducibility and stability of the biosensor was acceptable.
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