乙烯基功能化磁性分子印迹聚合物的制备和识别性能研究

摘要

In order to enhance the extraction and identification of polysaccharides in red pine bark extract, bifunctional monomers molecularly imprinted polymer microspheres (Fe3O4@SiO2—C=C@MIPs) were prepared by using magnetic Fe3O4@SiO2—C=C functional microspheres as the carrier, 4-vinyl phenylboric acid (VPBA) and acrylamide (AM) as functional monomer, N, N′-methylene double acrylamide (MBA) as crosslinking agent and ammonium persulfate (APS) as initiator for specific adsorption of starch polysaccharides.The properties of molecularly imprinted polymeric microspheres were determined by scanning electron microscopy, transmission electron microscopy and infrared spectroscopy.The results showed that APBA was successfully grafted on the surface, and the starch adsorption amount and the imprinting factor IF reached 12.48mg/g and 3.029, respectively.The adsorption of MIPs was more consistent with the second-order kinetic model and Langmuir adsorption curve.The covalent bond binding of boric acid derivative (APBA) and polysaccharide molecule and the hydrogen bond binding of amides compound (AMPS) and polysaccharide molecule increased the adsorption amount and specificity of molecular imprinting.Fe3O4 @SiO2—C=C could specifically adsorb G10000 polysaccharide.%为强化红松松皮中多糖的提取和识别, 以磁响应性的Fe3O4@SiO2功能性微球为载体, 4-乙烯基苯硼酸 (VPBA)和丙基酰胺基 (AM)为功能单体, 过硫酸铵 (APS)为引发剂, 制备了能够特异性吸附淀粉多糖的乙烯基双功能单体分子印迹聚合微球 (Fe3O4@SiO2—C=C@MIPs).通过扫描电镜、透射电镜及红外光谱确定分子印迹聚合微球性质.试验结果显示, 成功嫁接了APBA在其表面, 同时淀粉吸附量达到12.48mg/g, 印迹因子IF达到3.029.MIPs的吸附更符合动力学二级模型和Langmuir吸附曲线.硼酸衍生物 (APBA)与多糖分子的共价键结合和酰胺类化合物 (AMPS)与多糖分子的氢键结合提高分子印迹的吸附量和特异性吸附.Fe3O4@SiO2—C=C能够特性吸附松皮粗多糖中的G10000多糖.