Titanium surface was treated by means of different solutions (1wt% hydrofluoric acid (HF) solution, SOwt% hydrogen peroxide (H2O2) solution and HF/H2O2 mixed solution with ratios of 1:5, 5:1 and 1:5), and then obtained activated surface with different topologies. The structure, roughness, hydrophilicity and chemical properties on the surface of the samples were characterized and analyzed using scanning electronic microscopy (SEM), atomic force microscopy (AFM), contact angle meter and X-ray photoelectron spectroscopy (XPS). In addition, we also investigated how the treated titanium surface affects the morphology, proliferation and differentiation of osteosarcoma cells (MG63) by in vitro cell culture. The results show that the titanium surfaces treated by HF and H2O2 mixed solution are rougher and have more F- and OH- group than the others, indicating that these surfaces are more fit for the cell's adhesion, spreading, growth and proliferation. The titanium surface treated by HF and H2O2 mixture with 1:5 ratio presents the most excellent cell biocompatibility at the immediate time of cell culture. So, maybe the treating method in the study is effective to promote the rapid integration between titanium and its surrounding bone tissues.%采用不同比例的氢氟酸(1％(质量分数)HF)与双氧水(30％(质量分数)H2O2)以及二者不同配比(1∶1、5∶1和1∶5)的混合溶液对钛金属表面进行处理,制备出具有不同拓扑结构的钛活性表面.用扫描电镜(SEM)、原子力显微镜(AFM)、接触角测试和X射线光电子能谱(XPS)等手段分别对各组样品的表面形貌、粗糙度、亲疏水性及表面化学组成变化等进行分析和表征.将各组样品分别与骨肉瘤细胞株(MG63)共培养,用SEM观察细胞形态变化,利用MTT比色法测定细胞增殖能力,通过体外细胞培养实验考察处理后的钛金属表面对MG63细胞形貌及增殖分化特性的影响.结果表明,经HF和H2O2混合溶液处理后的样品表面粗糙,并含有丰富的F-和OH-基团,促进了细胞的粘附、铺展、生长和增殖,大幅改善了钛表面的生物活性.其中,氢氟酸与双氧水按1∶5配比的混合溶液处理后的样品在细胞培养前期显示出更加优良的细胞相容性,这对促进钛种植体与周围骨组织间的快速整合具有积极意义.
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