岩藻聚糖改性氧化钛与纤维蛋白原的界面反应研究*

摘要

The adsorption of fibrinogen can be used as a quick indication of surfacehaemocompatibility determi-nation because it’s prominent role in coagulation,in this study the molecular interaction between fibrinogen and modified titanium dioxide surface/platelet has been studied by quartz crystal microbalance with dissipation (QCM-D)and infrared spectroscopy (IR )in situ.These investigations revealed the fibrinogen have the trend to adsorb on hydrophilic surface in a side-on orientation by positively charged aC domains,which would reduce the exposure of platelet bonding site on gamma chain and enable less platelet adhesion and stimulated.%针对抗凝血生物材料与血浆关键凝血蛋白质界面作用机制欠缺问题，利用岩藻聚糖改性处理 Ti-O 薄膜材料，结合先进的原位界面表征技术 QCM-D (石英微天平技术)和红外光谱原位研究纤维蛋白原在其表面的吸附行为，确定其分子吸附及二级构象变化机制。进一步利用原位 ELISA(酶联免疫法)和 SEM (扫描电镜)从分子角度深入探讨了纤维蛋白原构象变化与凝血行为的关系。研究揭示了生物材料可以通过分子间作用力导致吸附纤维蛋白原出现不同的吸附形式，并通过影响其二级构象而导致血小板在材料表面出现不同的凝血行为，为抗凝血材料的优化设计提供了更为详尽的理论依据。