聚酰胺多孔膜的可控制备及细胞生物学响应

摘要

利用相转移法,通过控制溶剂蒸发温度制备了不同孔隙结构的聚酰胺(PA)多孔膜,用扫描电镜(SEM)和材料力学试验机对不同孔隙结构的PA多孔膜的形貌和力学性能进行了表征.将PA 多孔膜与类成骨细胞(MG63)共培养来考察不同孔隙结构多孔膜的细胞生物学响应.结果表明,随着溶剂蒸发温度升高,制备的PA多孔膜孔径大小逐渐增大,在40℃下制备的多孔膜孔隙结构均匀,孔径为10~20μm,其弹性模量与拉伸强度分别达到(20．2±0．9)和(5．2±0．5)MPa.细胞实验表明,与40℃下制备的PA多孔膜共培养后,细胞在其表面伸展爬行,形态结构完整,增殖明显.具有可控特性的 PA 多孔膜能够满足其在骨组织引导再生临床中的应用.%The porous polyamide (PA)membranes with different structure were prepared using phase-inversion technique by controlling the temperature of solvent evaporation.The morphologies and mechanical properties of the porous membrane were characterized by SEM and universal mechanical testing machine.The cytological properties of the porous membranes were studied in vitro by co-cultured with osteoblast-like MG63 cells.The results showed that the pore size of the PA membranes was increasing with the increasing of the treatment tem-perature.The uniform pores was obtained and the average pore size was 10 to 20μm when heated at 40 ℃.The elastic modulus and tensile strength reach (20.2±0.9)and (5.2±0.5)MPa,respectively.The cytological exper-iments demonstrated that the porous membranes displayed favorable cytocompatibility.After co-cultured with the PA membranes prepared at 40 ℃,porous membranes acted as a good matrix for MG63 adhesion,crawl and proliferation.The cytocompatibility,tunable mechanical properties,hydrophilicity of the porous membranes cauld meet the requirement of guided bone regeneration.